Byung Hun Lee

biorxiv.org/content/10.648… What is the computational role of dendritic excitations in vivo? We mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality.

There have been several impressive recent studies on dendritic voltage and calcium imaging in hippocampus. In this blog post, I review some of them: gcamp6f.com/2026/05/19/den… Great work by @BHL1117, @XiangWu96, Adam Cohen, @attila_losonczy, Balazs Rosza, Kevin Gonzalez, and others!

A 2025 Science study found that different dendritic segments of a single neuron follow distinct rules. The results challenge the idea that neurons follow a single learning strategy and offer a new perspective on how the brain learns and adapts behavior Learn more in this #SciencePerspective: scim.ag/4thRm1k #ScienceMagArchives

I started a company! Luminos. We made a complete neuro-electronic interface: stimulate and record from any set of neurons in any pattern of space and time, over a 6 mm field of view. Also useful for optical pooled screens and target discovery. luminosmicro.com

@Derek_Ran We used an ORCA Fusion CMOS camera. Many groups perform voltage imaging with two-photon microscopy, but photon yield is low yet and scanning at ~1 kHz is challenging. Sparse expression, structured illumination, and signal unmixing can help reduce background noise.

@BHL1117 CMOS camera can be used in vivo to image the voltage dynamic? how about two-photon

biorxiv.org/content/10.648… What is the computational role of dendritic excitations in vivo? We mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality.

@imalsogreg Sorry for the late reply. For sure the bigger neuron will have different dynamics. Even if their membrane properties are similar, longer dendrites should allow more independent computation. I would therefore expect more components, even in mouse L5 neurons.

@BHL1117 Amazing dataset and incredible finding! The low frequency of dendrite-initiated APs is so counterintuitive! I disbelieve your claim about 3 components being sufficient :) mouse pyramidal cells are small; are dendritic conductances in the much thicker rat and primate CA1s faster?

Thrilled to highlight the wonderful work of my colleague and close friend @XiangWu96 on voltage + Calcium imaging throughout the dendritic tree of hippocampal pyramidal neurons in navigating mice. He mapped the transfer function of voltage to calcium along the dendrites!

Dendrite-targeting OLM interneurons regulate the formation of learning-related CA1 place cell representations biorxiv.org/content/10.648… #biorxiv_neursci

@eunji_kkong Thank you!! 🙏

@BHL1117 Very exciting!!! Congrats👏

I feel very lucky to have worked with such an incredible team. I’m especially grateful to my postdoc mentor, @AdamEzraCohen, for his constant support and guidance—I’ve learned an enormous amount.

5. Dendritic biophysics shapes place field structure. Simple spikes and complex spikes distribute differently across place fields. Our data reveal the specific ion channel biophysics which set this biophysical “eligibility landscape” for plasticity during spatial coding.

Great one on Neurotransmitters