RNA

Telomeres are transcribed into TERRA. We showed that iTERRA is transcribed from interstitial telomeric repeats (ITSs) and that CpG island subtelomeric promoters are also present at interstitial sites bit.ly/4oD0UBH

Mutation of a conserved amino acid in the Prp8 alpha-finger increases use of non-YAG 3' splice sites in budding yeast and provides insight into Prp22 ATPase function. bit.ly/47YRb1g

m⁵C-Rol-LAMP, a simple qPCR-based assay, quantifies m⁵C RNA methylation at single nucleotides and uncovers stress-regulated rRNA modification in bacteria. bit.ly/43AWnr4 #LMU_muenchen #lifescimunich #RNA #CRC1309

Conserved protein Seb1 that interacts with RNA polymerase II and RNA is an anti-pausing transcription elongation factor bit.ly/4pbUQzQ

IsoformMapper creates protein networks from AlphaFold models and uses InfoMap community detection to compare isoform structures and visualize alternative splicing consequences via alluvial diagrams. bit.ly/49n4USa #AlternativeSplicing #RNASplicing

A genome-wide screen revealed that proteins lacking stable structures can be just as essential as folded ones. Among them, FAM32A plays a crucial role in RNA splicing. @UTokyo_News_en @KotaroTsuboyama bit.ly/4o7muy3

A PLP-RCA method enables simultaneous, in situ detection of AAV transgene DNA and mRNA at single-cell resolution, enabling the analysis of genome processing and transduction efficiency. bit.ly/4qoAR2g

By analyzing the subcellular localization of various UPF1 mutants, we show that UPF1’s nuclear-cytoplasmic shuttling is independent of its ATPase and RNA binding activities, with RNA binding itself being a key determinant of its cytoplasmic retention. bit.ly/3IFlQII

Molecular dynamics reveal how Mg²⁺, K⁺ and Gdm⁺ synergistically drive allosteric changes in the ykkC-I riboswitch aptamer, fine-tuning the RNA conformational landscape for function. bit.ly/4hgs9ze

NMR structures and ³J couplings combined with MD and maximum entropy refinement yield ensembles consistent with experiment, revealing dynamic conformations of Omicron s2m RNA. bit.ly/4nUTycB

DEAD-box protein Prp5 hydrolyzes ATP/ATPγS to drive prespliceosome formation. ATP is dispensable in the absence of Sub2, which is not essential, but facilitates splicing by stabilizing Msl5 binding. bit.ly/4nw3sB3

SpDis3L2-U13 RNA crystal structure further unveils its degradation mechanism. A single substitution at A756 disrupts Dis3L2’s ability to process structured RNAs, highlighting a key functional residue. bit.ly/4pTuJyO

Tiny ocean creatures called diplonemids bend the rules of genetics! Their genes are packed with unusual introns, yet their editing system works fine, showing the flexibility of life. bit.ly/3KUBOPC

This work highlights the conserved role of NMD in the degradation of lncRNAs in an RNAi-capable budding yeast and provides insight into the functional impact of endogenous RNAi on the transcriptome. bit.ly/3IvOmwj

Crystal structure of the LIG-KIN domains of fungal tRNA ligase Trl1 reveals interdomain assembly and RNA transfer model, offering insight into a possible antifungal target. bit.ly/3IZ7SBn

Loss of a base (A3027) in the sarcin/ricin loop (PDB 4V7R) of 25S rRNA is sensed by factors detecting slow-moving ribosomes. Ubiquitination of the small subunit occurs, despite large subunit damage. bit.ly/4pRJfHD

Desthiobiotin (DTB)-modified and TAMRA-modified 2''OMeNAD+ are RNA 2'-phosphotransferase (Tpt1) poisons that enable affinity-tagging and fluorescence-tagging of internal RNA 2'-phosphate groups. bit.ly/46dMUHZ

The #RNA editing enzyme ADAR1 is an important target for many cancers. This study evaluated ZYS-1, a recently reported ADAR1 inhibitor, and found no evidence supporting ZYS-1 as an ADAR1 inhibitor. bit.ly/4nEK7xc

T-box-i is a novel, effective inhibitor of T-box riboswitches with antimicrobial activity against Staphylococcus aureus. bit.ly/4nyMmSE

HPV E6 exploits codon usage to reduce antigen presentation and evade immunity; combinations of synonymous substitutions restore E6 expression and T cell activation. bit.ly/3VUKzvo