Fabien Le Grand

With @GilbertLab , we designed a pipeline to study communications toward muscle stem cells during tissue regeneration. How do MuSCs re-quiesce? Check the work by our star students @GarciaPaulineJ & @Erik_Jacques_ in our new preprint. biorxiv.org/content/10.110…

@nathangburns You could not find a better place to read our paper 😃

I am delighted to share our newest paper. @GarciaPaulineJ found that Setdb1 is absolutely required for muscle tissue regeneration. This by keeping ancient viral elements silenced in muscle stem cells. @EJPRareDiseases @iNeuroMyoGene @AfmPresse

@vaiter Je suis dans la même situation, très anxiogène vu le travail investi avec les partenaires PRC. Une réponse @AgenceRecherche ?

J'ai un dépôt ANR de Schrodinger🐈. J'ai un email qui accuse réception de ma soumission, et un autre email qui me dit que ma soumission ne sera pas évalué car pas reçu. À moins que cela soit une soumission normande🤨?

@AgenceRecherche j'ai recu, mercredi, mail de confirmation de votre part de l'enregistrement de notre pre-proposition PRC. Hier je reçoit un mail prétendant une "absence de confirmation", que notre projet ne sera pas considéré. Veuillez expliquer ces contradictions, je vous prie.

@DumontN_Lab @NatureComms Bravo Nicolas et Collègues ! 🔥

I’m beyond proud of this manuscript published in ⁦@NatureComms⁩. We identified a mechanism behind muscle stem cell defects in DM1 and demonstrated that we can target these defective cells to restore myogenesis. nature.com/articles/s4146…

@mick17000 A great suggestion! Let me run it through @EpigeneticsYOW , and see what the chairman thinks of it 😁

@LeGrandLab1 You should give your talk by the swimming pool 😉

Delighted to share our latest work on BioRxiv. During her PhD, @GarciaPaulineJ relentlessly studied the impact of removing the H3K9 methyltransferase Setdb1 in muscle stem cells. Strikingly she found Setdb1 is absolutely required for regeneration 1/n biorxiv.org/content/10.110…

This work will be presented live, next thursday at the GRC Myogenesis meeting. If you are attending, you should read the preprint so you may (1) Ask well thought-after questions or (2) Skip my talk and go swimming/running instead 😉

Read our preprint, and send us your feedback 😃. We deeply thank to the funding by @EJPRareDiseases @Telethon_France and our collaborator @t_cheung .

Un poste de gestionnaire financier est à pourvoir au sein de l'INMG. Découvrez l'annonce ci-dessous 👇

You asked for it #myotwitter and @Erik_Jacques_ delivered! A new 3D assay to study regulators of muscle stem cell quiescence and niche repopulation principles 'in a dish'. Our easiest method YET! What questions will you ask?

Check out Nicolas Rose study, out in Biomaterials. He navigated between three labs/co-supervisors during his PhD, and yet successfully created very tiny 3D myotubes between pillars. This is a new tool to measure the force generated by a single human muscle cell in health/disease.

@Mic_Hicks @apkann I wash and stain fibers in wells of a 48-well plate. I let them sediment, no spinning. Also, at the beginning, I was loosing quite a lot of fibers during plating on the microscope slide (stuck to the glass pipet). I found that doing the last wash with PBS+5%serum really helps.

@apkann I have been reading up on the Goel/Krauss book chapter :). Do you spin between washes or just let the myofibers settle? Is it hard to transfer the myofibers from the FACS tube to the microscope slide wo losing yield?

Trying my hand at single myofiber isolation. What are some tips for immunostaining?

@DumontN_Lab @BentzingerLab @vahabsoleimani @chang_natasha Invite me over next time! I am not so far away 😁

Very interesting day discussing muscle stem cell biology with the labs of @BentzingerLab @vahabsoleimani @chang_natasha and Colin Crist. Great work by the students presenting their projects.

@AntoineZalc So, did you get your head checked? By a jumbo jet? 😁

Song 2 is definitely my walk to the stage song when this becomes the norm at conferences 🤪

@jgryall I am having a great time, indeed.