Yusuke Nasu

Today we report single-cell APEX-seq (scAPEX-seq) — a new method for unbiased mapping of *subcellular* transcriptomes at single-cell resolution. This approach reveals cell states that are not detectable by standard scRNA-seq, and enabled us to identify regulators of CAR T function that improve solid tumor killing. biorxiv.org/cgi/content/sh…

Congratulations to Dr. Makoto Shirakawa @MakotoShirakaw2 on receiving the JSPP Young Investigator Award! The award ceremony was held on March 14 in Tokyo.

Excited to share our new paper on GRAB_PGE2-1.0, the first genetically encoded fluorescent sensor for monitoring spatiotemporal prostaglandin E2 (PGE2) dynamics in vivo! It enables real-time imaging in cultured cells, acute brain slices, and living mice.

私の所属する研究所が４０周年を迎えるにあたり、関連分野のリーダーを招いてシンポジウムを開催します。定量生物・生物物理関連では James Ferrell, Michale Elowitz, Wendell Lim らが登壇してくれます。三日間の濃密なプログラムです。ぜひお越しください。

Excited to share our new preprint with @fbayashi_T! We developed a boosted PURE-based cell-free FADS platform for rapid protein evolution. Using this platform, we evolved highly robust SP6 RNA polymerases and engineered split variants that sense diverse biomolecular interactions.

Our collaborative study with Yasui lab @sciencetokyo_ja is out in @ScienceAdvances ! With gRNA/GeNL tagging, we showed that cancer-derived small EVs can reach and be concentrated in urine via transcytosis, providing rationale for urinary liquid biopsy. science.org/doi/10.1126/sc…

羊土社『実験医学』3月号にて、Academia Sinicaおよび私の研究室の紹介記事を寄稿しました。研究環境や日々の取り組み、Academia Sinicaで研究を行う魅力についてまとめています。ご関心のある方にお読みいただけましたら幸いです。 Academia Sinicaでは各Institute（大学の学部に相当）でほぼ毎年PIの公募が行われていますが、それに限らず、大学院生やポスドクとして挑戦できる機会も数多くあります。近年は、欧米でポスドクをされている日本人研究者や、日本で研究を進めてこられた方々の着任も増えており、日本との距離も年々縮まっていると感じています。 本稿が、学生・ポスドク・PI志望の方を含め、海外での研究という選択肢を考えるきっかけになれば嬉しく思います。

📢 Applications are open! Join us @HHMIJanelia to explore next-gen fluorescent proteins, biosensors, imaging advances + ML/computational approaches to accelerate design. 🛏️ Meals & lodging covered Apply by May 21 ➡️ janelia.news/FPS26 @HHMIscience @ERSchreiter @agtebo

Come join us at the Quantitative Microbiology Symposium this May in Taipei! We’ve invited outstanding researchers working at the interface of the mathematical sciences and microbiology. For details and registration, see: sites.google.com/view/q-microbi…

🧪🧬 New preprint! What if the same biosensor could reveal how cysteine is regulated across life domains? We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells. biorxiv.org/content/10.648… 🧵A thread ⬇️

A new kalium channelrhodopsin from our collaboration with Wan-Chen Lin's lab! @IBMS_AcadSinica Strong suppression of axonal excitation and glutamate release from the long-range CA3 input for the critical tests at our hands :-) biorxiv.org/content/10.648…

【拡散希望】
４月から東北大学際研の助教として新規チームを立ち上げるにあたり, 学振研究員の受け入れを始めます. アクチンで人工細胞を走らせたい方, 未培養生物の細胞骨格の無細胞合成に興味のある方、ご相談ください. そんなのつまんねーから○○で申請したい, という強気な提案も歓迎です.

年の瀬ギリギリに論文が公開されました！ Our paper regarding "A Nonviral Neo-Nucleocapsid for Cell-Specific RNA Delivery Developed by Pseudo-Cyclic Peptide Grafting and Directed Evolution" has been pubilished in Angewandte Chemie! onlinelibrary.wiley.com/doi/10.1002/an…

TAIWAN BRIDGES〉Launches at Academia Sinica Nobel Laureate Roger D. Kornberg Outlines Scientific Solutions to Pandemics sinica.edu.tw/en/news_conten… #TaiwanBridges #AcademiaSinica #NobelLaureate #PandemicSolutions

(1/6) Excited to share our new bioRxiv preprint with H. Sakamoto (UTokyo)—my first paper started after returning to Japan. DOI: biorxiv.org/content/10.648…

New work describes our efforts to achieve CRISPR editing of the mitochondrial genome. biorxiv.org/lookup/content… The CRISPR toolbox has revolutionized the study of nuclear DNA, but the mitochondrial genome (mtDNA) has remained out of reach, mainly because there are no known ways to deliver sgRNAs across both outer and inner mitochondrial membranes. Yet many organisms have evolved mechanisms to transport RNAs across their mitochondrial membranes. Trypanosomes, for example, do not encode any tRNAs in their mtDNA, and must import all of them from the cytosol to support intra-mitochondrial protein translation. We started with yeast. We engineered a strain that must repair a STOP codon in mtDNA (an arginine biosynthesis gene) to survive. After screening a library of sgRNA import sequences, we identified one that enables a small degree of functional CRISPR editing in mitochondria. Graduate student Sifei Yin obtained these exciting results several years ago, and spent the intervening time understanding how sgRNA import works, and how to make it better. Read the paper to learn what she uncovered!

【研究員募集】 東北大学、松林研究室では、博士研究員を募集しています。 人工細胞を走らせたい方、細胞骨格機能のエンジニアリングや起源の探究などのテーマに関心のある方、ぜひご検討ください！ fris.tohoku.ac.jp/recruit/invita… jrecin.jst.go.jp/seek/SeekJorDe…

【ポスドク募集】 代謝・神経活動を可視化する機能性タンパク質／バイオセンサーの開発に挑む仲間を募集しています。 protein engineering・イメージング技術が好きな方、ぜひご連絡ください！ #ポスドク募集 #Postdoc #ProteinEngineering #Biosensor #FluorescenceImaging #Metabolism

David Baker研の本田信吾さんにセミナーをしていただけることになりました！　皆様ぜひご参加ください。 「タンパク質でオリガミする」 Computational design of highly addressable protein assembly 2025年12月11日 (木) 16:00～ 東京大学 駒場Ⅰキャンパス 16号館107会議室

Our new paper is out in GLIA! We show that astrocytic Igfbp2 in sclerotic hippocampus promotes spontaneous seizures. The dataset may also reveal new glial targets for future antiepileptic therapies. 🔗 onlinelibrary.wiley.com/doi/10.1002/gl… #astrocyte #epilepsy