Neal Callaghan

@DrPietvanVliet Let us know how it goes!

@TheSurajKannan The disease modeling literature reflects perturbed membrane-associated proteomes, PTM states and splicing, whether causative or secondary to the altered homeostasis. Would be great to start querying these in PSC-CM maturation, but in vivo controls for proteomics are tough.

@TheSurajKannan This is in clear contrast to in vivo CMs, which your lab has demonstrated to have a much more evident trajectory toward "maturity".

This is a really data-rich contribution to the PSC-CM maturation literature. A lot of maturation papers are unfortunately haphazard but this one is thoughtful in its iterative optimization and rationale behind the tested media components. Thorough functional measurement as well.

Thanks to @craigsimmons11, @seema_mital, @milicaruoft, @FilioBillia, and all the other coauthors @trogersresearch for the wildest PhD project I could have imagined! (20/20 = I'm done now) (cyan DAPI/nuclei, magenta SERCA2a, yellow RyR2)

Want to try C16 in your own lab? Go crazy! The recipe is in the preprint. Drop us a note if you want to chat more about how we use it, or how it was developed. (19/20)

Excited to share our new preprint coming out of @TBEP_UofT, where we demonstrate enhanced physiological maturation of stem cell-derived cardiomyocytes (heart muscle cells)! biorxiv.org/content/10.110… (1/20)