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Josh Selfe
30 posts
Josh Selfe
@joshsselfe
Neuroscience PhD student in the Akerman lab @OxNeuro. Intrigued by cortical circuits, all-optical techniques, sensory processing.
Oxford, United Kingdom Beigetreten Haziran 2011
114 Folgt44 Follower
Josh Selfe retweetet
Our paper "Memory reactivation generates new, adaptive behaviours that reach beyond direct experience" is now out #Abs1" target="_blank" rel="nofollow noopener">nature.com/articles/s4159… ! With @AnnieBRawson, Jill O’Reilly and @HelenCBarron at @MRCBNDU @NDCNOxford 1/6
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@kiradusterwald Thanks for the awesome modelling Kira and @EranShorer!
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Exciting to see this out after working on the modelling with @EranShorer! 🌸 ORCHID = all-Optical Reporting of CHloride Ion Driving force & uses voltage imaging with a Cl-permeable channel to evaluate inhibitory transmission in neurons. Epic effort @joshsselfe & @JosephRaimondo!
Josh Selfe@joshsselfe
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms nature.com/articles/s4146…
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Awesome to see this finally out! Congratulations @joshsselfe and @JosephRaimondo!
Josh Selfe@joshsselfe
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms nature.com/articles/s4146…
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Congrats @joshsselfe and the team!
Josh Selfe@joshsselfe
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms nature.com/articles/s4146…
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So cool! Congratulations @joshsselfe 👏🤩
Josh Selfe@joshsselfe
Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms nature.com/articles/s4146…
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Huge thanks to @Abdelfattah_Lab, @ERSchreiter, and @rhodamine110 at @HHMIJanelia, and to @RichBur, Sarah Newey, and Colin Akerman at @PharmOxford for all of the help and support along the way! And to @MPrag for the photo of the beautiful orchid 📷 (7/7)
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snRNAseq by Teresa Steyn, computational modelling by @EranShorer and @KiraDusterwald, and IHC by @AKraitzick. All with @UCT_HUB, @UCT_NI, @UCTIDM, and @UCTHealthSci. (6/7)
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Ever wanted to explore the driving forces underlying inhibitory neuronal signalling? We did! So, for my MSc in @JosephRaimondo lab, we developed the all-optical approach ORCHID - all-Optical Reporting of CHloride Ion Driving force. Now out in @NatureComms nature.com/articles/s4146…
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ORCHID is additionally advantageous in small cells or subcellular compartments, and opens the doors for using all-optical methods to assess driving force more generally. (5/7)
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We use ORCHID to confirm the mechanisms that establish Cl- driving force, reveal novel differences between neurons and astrocytes under different network states (1), and make the first in vivo measurements of intact Cl- driving force in mouse cortical neurons (2). (4/7)
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Amazing work by @gsgothard showing the developmental origins of the local inhibitory subnetworks of L2/3 pyramidal neurons. Give it a read!
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Super excited to share that this preprint is now out, the result of my MSc, and the first all-optical reporter of ionic driving force!
Joseph Raimondo@JosephRaimondo
We are excited to present ORCHID, a new tool for all-Optical Reporting of Chloride Ion Driving force. bit.ly/45P6est In the brain, inhibition is mediated by Cl- permeable receptors. ORCHID can therefore be used to quantify the driving force across inhibitory receptors.
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