Plate Lab

Lastly, is MLEC important for SARS-CoV-2 replication? We use a replicon system to show that YES, it is! (7/7)

MLEC surveys our glycoproteins during production, checking for errors and capturing duds before they are sent out. Is this function of MLEC important for CoV replication? Yes! (6/7)

Excited to share a preprint from @J_P_Davies PhD work! We find that CoVs, like SARS-CoV-2, use malectin (part of cells’ glycoprotein production assembly line) to make viral proteins and replicate. Malectin may be a prime pan-CoV antiviral target! 🧵(1/7) biorxiv.org/content/10.110…

Congratulations @EliFritzMcD !

Good morning Phoenix. Looking forward to attending #NACFC2023. If you’re around, come to the lunch roundtable on Proteomic-driven Advances in Studies of CF (RT29) where we’ll discuss the latest mass spec research on CF. @CF_Foundation

In sum, AM predicts CF accurately w/ a high false positive rate. AM correlated modestly w/ clinical outcomes, but well w/ CFTR function. It cannot distinguish pathophysiology. AM may prove useful for identifying CF causing variants in carriers, but caution must be taken. (7/7)

Finally, we were curious if AM could predict CFTR theratype - how CFTR variants respond to drug. This feat is technically outside AM's intended design, but is important in the CF field. We concluded, AM cannot predict CFTR theratype. (6/7)

Can #AlphaMissense identify Cystic Fibrosis causing variants? We benchmarked AM pathogenicity predictions on CF clinical outcomes from the CFTR2.org database and CFTR in vitro data. @EliFritzMcD @MeilerLab Here's what we found: 🧵(1/7) biorxiv.org/content/10.110…

Congrats @Dongsheng_Han1 and @JaredNordman! Glad that @J_P_Davies and our lab could contribute to this exciting paper.

Follow @J_P_Davies’s research activities in Peru:

@J_P_Davies from our lab is forging new research collaborations at @IINPeru in Lima! Thanks to @vuglobalhealth and @VI4Research for spearheading this new program!

We concluded translational dynamics modulate the co-translational folding of P67L sufficiently to sensitive it to VX-445. Personalized medicine will need to account for system level changes beyond single point mutations in targets.