This may be one of the most revealing admissions in modern virology. If a field has worked "without virus isolates forever," then it has spent decades assuming the existence and properties of the very thing it claims to be studying. That's not validation—it's circular reasoning.
@ViroLIEgy@MarionKoopmans Now I know you have no idea about MS besides no having a clue about virus. It was YOU who proposed to use MS. Thank you for demonstrating you're totally unaware of of your ignorance.
@ManiKeops@MarionKoopmans 3. "Mass Spectrometry:" Running an MS on an unpurified, multi-component mixture just gives you a chaotic readout of host proteins. It tells you absolutely nothing about a specific "pathogen" because you have no pure baseline to compare the data against.
@ViroLIEgy@MarionKoopmans Wrong. isolation and purification: read and don'r embarrass yourself. Stop using jargon you don't know about. Learn about limits of fhost cell protein and how, purification steps work. Read
@ManiKeops@MarionKoopmans 2. "TEM:" TEM confirms homogeneity. If your TEM grid shows a chaotic mixture of varying cellular debris, microvesicles, and unidentifiable blobs, it proves you are looking at a contaminated mixture, not an isolated variable.
@ViroLIEgy@MarionKoopmans Nice, but wrong. I could start commenting on clarification, centrifugation, ultrafiltration TFF or chromatographic purification steps... that prove that you are repeating moronic bullshit.
Read and stop bothering.
@ManiKeops@MarionKoopmans 1. "Multistep purification:" It does not matter if you have a 2-step or a 50-step process. If the final step still leaves your purported "virus" floating in a soup of host-derived extracellular vesicles, you have not achieved physical purity or established an IV.
@ViroLIEgy@MarionKoopmans Running a MS on viral sample, "Physical purity", total and blatant ignorance on multiple step purification processes, thinking that TEM can show absence of contaminants which are relevant to viral seed behaviour. And "I've read plenty": posts on X, maybe, scientific texts, none
@ViroLIEgy@MarionKoopmans You think you've read plenty, and it may have involved a great intellectual effort, but you are not even grasping characteristics of the materials you pretend to know about. Lack of knowledge is such you cannot even measure your ignorance. And yes, your texts are showing that
@ViroLIEgy@MarionKoopmans Read a f*ckin text on virus isolation and purification and stop bullshit about "biochemical analysis, physical purity and MS".
You ar talking nonsense.
@ManiKeops@MarionKoopmans Insults show that you know that you lost the debate and that you have no logical counterargument. Any more insults, and your time is done.
@ManiKeops@MarionKoopmans Thanks for listing the exact blueprint of your field's scientific failure. You keep posting the screenshot because you cannot explain how you run analytical chemistry on a dirty, confounded mixture and call it "isolation." You are trapped. 👍
Dos personas admirables, valientes, resilientes, responsables, a las que damos las gracias por no dejarnos en manos de la purria FRANQUISTA y GOLPISTA.
Vamos a votar a este hombre con mayoría absoluta, para que pueda limpiar de fascismo este país.
Y le damos las gracias a Begoña
@Manolotabaiba1@EsterMunoz85 El TOP franquista de 1976, suponiendo que se sea TOP con 35 años (dudoso...), en 2026 tendría 85 años.
Pero, sí es posible que el TOP franquista de la guardería hoy sea magistrado en el supremo. Siempre es posible.
🔸️Primera imagen, 11:00 am : Península bloqueada por la geoingeniería climática para poner una barrera invisible y no llueva.
🔸️Segunda imagen, hora actual 17:20 pm: la geoingeniería climática ha cobrado el peaje de hoy y permiten que llueva en la mitad oeste peninsular
🤣
@ViroLIEgy@MarionKoopmans Whatever. Blatant ignorance and total lack of self awareness. People with minimal knowledge will realize just by reading your nonsense. But you're not yet there.
@ViroLIEgy@MarionKoopmans Read a basic text and stop bothering. It is like dillucidating molecular structure by using pHmeter. Crass ignorance does not allow you to realize how dumb it is.
@ViroLIEgy@MarionKoopmans Paradigmatic Dunning-Krugger. You did it yourself.
Your ignorance is so immense, you cannot realize.
There are good texts, free access, about viral harvesting, isolation and purification. Go and learn.