Florian Hoffmann, PhD

70 posts

Florian Hoffmann, PhD

Florian Hoffmann, PhD

@fthoffmann

Postdoctoral Scientist @Stanford • Ph.D. @Columbia @sternberglab • @UCL Bichemistry ‘20 • Transposons, microbes, viruses, CRISPR genome engineering

Stanford, CA Katılım Aralık 2019
337 Takip Edilen290 Takipçiler
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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Excited to share my last major PhD work from the @SternbergLab. We discovered a mechanism that initiates transcription at programmable sites, without requiring promoter sequences - a novel mode of transcription regulation in bacteria.
Sternberg Lab@SternbergLab

New pre-print(s) from the Sternberg Lab in collaboration with @LeifuChangLab! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: tinyurl.com/mr3fd493

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Florian Hoffmann, PhD retweetledi
nature
nature@Nature·
Nature research paper: Exapted CRISPR–Cas12f homologues drive RNA-guided transcription go.nature.com/3P0wiNu
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nature
nature@Nature·
Nature research paper: Structural basis of RNA-guided transcription by a dCas12f–σE–RNAP complex go.nature.com/3MXvicv
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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Our 2 papers on RNA-guided transcription are now out @Nature. This mechanism re-writes the traditional concept of bacterial transcription and allows RNA transcripts to be generated de novo from potentially any cellular DNA sequence. 🧬 See below for links and thread 🧵
Sternberg Lab@SternbergLab

Out now! In collaboration with @LeifuChangLab, we uncover the molecular and structural underpinnings of CRISPR-Cas12f-like RNA-guided transcription systems! Links to the articles in the following tweet:

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Jacopo Gabrielli
Jacopo Gabrielli@jacopo_gab·
Certified Alien 🇺🇸👽
Jacopo Gabrielli tweet media
San Francisco, CA 🇺🇸 Français
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Simone Alma Evans
Simone Alma Evans@simone_alma·
I am so excited to share our project with you! We find prokaryotic proteases activate toxic enzymes and pores as a modular strategy in phage defense. We studied four fascinating protease-toxin pairs that are abundant across bacterial genomes: Many thanks to our wonderful collaborators and to the Gao lab for making this work possible! biorxiv.org/content/10.110…
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Max Wilkinson
Max Wilkinson@maxewilkinson·
The Wilkinson Lab is open for science! @MSKCancerCenter 🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
Max Wilkinson tweet media
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Florian Hoffmann, PhD retweetledi
Stephen Tang
Stephen Tang@stephentang23·
Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. doi.org/10.1101/2025.1…
Stephen Tang tweet media
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Ben Adler
Ben Adler@ben_a_adler·
Thank you to everyone who helped make this story happen! Obligatory meme to summarize the paper: “How I imagine phage facing CBASS and mCpol”
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Ben Adler
Ben Adler@ben_a_adler·
Today in @Nature , we highlight how a cousin of CRISPR-Cas10, mCpol, establishes an evolutionary trap in anti-phage immune systems. Check out Erin Doherty's and my work from Doudna lab here: nature.com/articles/s4158…
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Ramesh Wigneshweraraj
Ramesh Wigneshweraraj@wig_r·
@SternbergLab @LeifuChangLab This is a wonderful story. Changes the paradigm underpinning how bacteria can initiate transcription. I wonder whether RNA dependent initiation of transcription is co-opted by phages…..
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Sternberg Lab
Sternberg Lab@SternbergLab·
New pre-print(s) from the Sternberg Lab in collaboration with @LeifuChangLab! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: tinyurl.com/mr3fd493
Sternberg Lab tweet media
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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Excited to share my last major PhD work from the @SternbergLab. We discovered a mechanism that initiates transcription at programmable sites, without requiring promoter sequences - a novel mode of transcription regulation in bacteria.
Sternberg Lab@SternbergLab

New pre-print(s) from the Sternberg Lab in collaboration with @LeifuChangLab! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: tinyurl.com/mr3fd493

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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
In parallel to our RNA-guided transcription biology story, we collaborated with @LeifuChangLab and determined how Cas12f interacts with a Sigma factor to recruit RNA polymerase. See their thread on the magnificent dCas12f-Sigma-RNAP structure below:
Leifu Chang@LeifuChangLab

Our new preprint is live! doi.org/10.1101/2025.0… In collaboration with @SternbergLab, we determined the cryo-EM structure of a dCas12f-σE-RNAP complex from F. taeanensis, revealing an RNA-guided transcription mechanism that bypasses canonical promoter motifs.

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Sternberg Lab
Sternberg Lab@SternbergLab·
Know the feeling when two things look similar, and yet turn out to be quite different? Seems like defense-associated reverse transcriptases will continue to surprise us... Another thrilling story uncovered in collaboration with @WiedenheftLab! Check it out:
Stephen Tang@stephentang23

The sequel to our DRT2 story is here, and this one is once again full of surprises! This time around, the @SternbergLab teamed up with the @WiedenheftLab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: biorxiv.org/content/10.110…

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Lena Steckelberg
Lena Steckelberg@LenaSteckelberg·
Here we present the first structure of a plant-virus xrRNA in its active, nuclease-resistant conformation. The RNA forms a pseudoknot that creates a protective ring around the RNA. This ring is very similar to that of flavivirus xrRNAs, despite lacking sequence similarity!
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Stephen Tang
Stephen Tang@stephentang23·
Thrilled, and also in a bit of disbelief, to see our paper featured on the cover of @ScienceMagazine! "Finding Neo" wasn't easy, but we just kept swimming (read: stared at a sequence of DNA) until the Eureka moment hit.
Science Magazine@ScienceMagazine

The central dogma of molecular biology states that genetic information flows from DNA and RNA to protein, with reverse transcription converting RNA to DNA. In the pursuit of understanding how bacteria defend themselves from viral infection, two groups have found alternative pathways to making genes from RNA that did not previously encode proteins. Learn more this week in Science: bit.ly/3ZO7tI1

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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Huge congratulations to the most brilliant scientist I‘ve met so far - @shsternberg. Hard to come across a more dedicated PI and mentor - I can‘t even imagine the amazing discoveries this will enable you and the whole @SternbergLab to make in the future! 🧬 🎉
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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Preprint alert 🚨 from the @SternbergLab ‘De novo gene synthesis by an antiviral reverse transcriptase’: new central-dogma-expanding work led by @stephentang23 is now online! You won’t want to miss this thread: biorxiv.org/content/10.110…
Stephen Tang@stephentang23

I am delighted to share the newest work from the @SternbergLab, in which we discover RNA-templated gene creation as a mechanism of antiviral immunity. This is a story for anyone who loves molecular biology, and one that repeatedly left us in disbelief. doi.org/10.1101/2024.0…

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Florian Hoffmann, PhD
Florian Hoffmann, PhD@fthoffmann·
Not a single week without a new paper from the @SternbergLab: led by bioinformatics star @TannerWiegand Bacteria use ancestral CRISPR-Cas genes as transcription factors!#crispr Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleases
Sternberg Lab@SternbergLab

New pre-print from the Sternberg Lab! Transposon-encoded nucleases (TnpB/IscB) gave rise to the CRISPR-associated proteins (Cas9 and Cas12), but were TnpB-like proteins domesticated for other functions? 𝙔𝙀𝙎!! doi.org/10.1101/2023.1…

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@Nature @jacopo_gab @simone_alma @maxewilkinson @bradyfcress @MSKCancerCenter @ben_a_adler @wig_r