Monack Lab

Very proud to see Alvin's work end up as the cover story for this months issue of @LA_NatRes nature.com/laban/volumes/…

Great to see this great work from Ruth Schade finally published and thanks to all funding sources and all of our collaborators @Salmonella

Thrilled to announce that we have another paper out today in @SciReports showing how intracellular Salmonella Typhi infection alter the transcriptional landscape of human macrophages. nature.com/articles/s4159…

We thank all our collaborators & co-authors for their contributions - and many others for reagents and feedback on our work!

SalComFit: We have published these thousands of fitness effects in a new #SalCom online resource bioinf.gen.tcd.ie/cgi-bin/salcom… The Gene Fitness Compendium developed in collaboration with Karsten Hokamp, Ada Luo @LijuanAda & Jay Hinton @Jay_Salsa.

Finally, we experimentally perturbed several serovar-specific gene networks & identify novel #pseudogenes in Typhi & Paratyphi A The pseudogenes sensitize human-restricted Salmonella to stress - including metal homeostatic pathways

We extend this pipeline to investigate how different Salmonella serovars infect human THP-1 macrophages. We identified genes ONLY required during infection by Typhi & Paratyphi A = NOT required by Typhimurium.

To systematically characterize these fitness events, we used @abarysh’s SAFE & other systems cofitness approaches to identify serovar-specific gene networks We identified several gene clusters that ONLY have phenotypes in Typhi & Paratyphi A.

We use Rb-Tn-seq to identify all genes used by Salmonella Typhimurium, Typhi & Paratyphi A to respond to 25 infection-related stresses encountered through human infection = thousands of fitness effects !! Rb-Tn-seq developed by Adam Deutschbauer & colleagues @Berkeleylab

We are thrilled to reveal our story in @NatureGenet on using high-throughput functional genomics to systematically study the biology of both generalist & human-restricted Salmonella: nature.com/articles/s4158… This work was led by our postdoc @BenjaminXWang1 #Salmonella

Check out our latest paper on how temperature influences gut motility and stress in mice. Kudos to Alvin Han for all his hard work and thanks to all of our collaborators for making this paper happen! nature.com/articles/s4168…

We went for a lab-excursion to the CSHL microbial pathogenesis and host responses seeing a beautiful talk by Blanda on her Treg story, while Joy, Ruth and Daniel presented posters on their exciting stuff.

Congratulations to Alvin Han in the lab for successfully (and eloquently) defending his thesis work 👏👏👏.

Correction, Bens talk is on Thursday...

Denise and Benjamin Wang are currently enjoying Italy at the GRC Salmonella conference. If you're around, make sure to go see Denise's beautiful poster and also to listen to Ben's exceptional story last thing Friday!! #Salmonella #Typhi #GRCSalmonella

Trung Pham and Denise published a seminal review on how intracellular bacteria rewire macrophages and creates a permissive niche for bacteria to thrive in. sciencedirect.com/science/articl… #Salmonella #Macrophages #Stanford #microbiology

Another successful thesis defence from Oscar Diaz and a soon to be ready manuscript that will hopefully pop up within a couple of months. #Science #Stanford #Salmonella

Lots of things has happened at the Monack lab over the last year! #Salmonella #Stanford #Science