Moffitt Laboratory

Excited to share our new work from Rosalind Xu, where we used MERFISH to build a single-cell spatial transcriptomic atlas of the mouse gut—and to explore host-microbiome interactions in situ! biorxiv.org/content/10.110… 1/

Interested in cutting-edge work in both the development and application of techniques to better understand gene regulation from a systems perspective? Abstract submissions are still open for this CSHL meeting: meetings.cshl.edu/meetings.aspx?…

Taken together, these results underscore the potential of our new murine gut atlas to inform a broad range of biological and biomedical questions! 12/12

Remarkably, many aspects of the gut were largely unchanged! Nonetheless, we observed profound shifts in immune cell abundance and distribution—highlighting the central role of the microbiota in shaping and educating the immune system. 11/

Excited to share our new work from Rosalind Xu, where we used MERFISH to build a single-cell spatial transcriptomic atlas of the mouse gut—and to explore host-microbiome interactions in situ! biorxiv.org/content/10.110… 1/

Yesterday our first grad student -- DR. Rosalind Xu -- had her official PhD hooding ceremony in @HarvardCCB! A massive congratulations to her and all of the other freshly minted PhDs!

As plasma cells play fundamental roles in tissue behavior in health and disease, we are excited to see the wide range of questions that BCR-MERFISH will now allow us and others to address! 9/9

To our surprise, we found that some plasma cell clones form patches of co-occurring clones along the gut, suggesting, perhaps, that there is local production of IgA specific to a subset of the microbiome! 8/9

Proud to announce our new technique for tracking relative B cell clonality in situ with B Cell Receptor MERFISH (BCR-MERFISH)! Congratulations to Evan Yang and our colleagues in the Carroll laboratory! Check out our bioRxiv: biorxiv.org/content/10.110… 1/9