Wayland Cheng Lab

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Wayland Cheng Lab

Wayland Cheng Lab

@WChengLab

Biophysical and structural studies of ligand-gated ion channels. Lipid modulation. @WUSTL_AnesRsrch Washington University Department of Anesthesiology

Washington University Sumali Ağustos 2020
431 Sinusundan620 Mga Tagasunod
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Wayland Cheng Lab
Wayland Cheng Lab@WChengLab·
Our study on nanodiscs and ion channel structure is now published: rdcu.be/duXkK. We hope this will spur more consideration into the impact of nanodiscs on membrane protein structure. @VikramDalal_37 @PageAnesthesia @GraceBrannigan @WUSTL_AnesRsrch
Misha Kudryashev 🔬 ❄🕊️@MishaKudryashev

This is a very interesting paper showing that nanodiscs, typically used to preserve lipids for structural analysis of membrane proteins, can affect the structure of the target protein. First paper I read this year, happy 2024! nature.com/articles/s4146…

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Baron Chanda
Baron Chanda@ChandaLab06·
Structures of channels in Open conformation are rare. Thrilled to share our latest manuscript.. Structural basis for hyperpolarization-dependent opening of human HCN1 channel disq.us/t/4p78xec
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Dastvan Lab
Dastvan Lab@DastvanLab·
We are grateful to have received an equipment supplement for our @NCI R37-CA265877 grant. This support will significantly enhance our research capabilities. Thank you @NCICancerBio٫ @amin_nci.
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WashU Medicine Anesthesiology Research
WashU Medicine Anesthesiology Research@WashUAnesRsrch·
Jenna Lin, a graduate student in the @ChandaLab06, received the prestigious 2024 John E. Majors Award for her outstanding accomplishments in research and teaching! Congratulations, Jenna!
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Filip Van Petegem
Filip Van Petegem@FilipPetegem·
A postdoc position is available in our lab to study how ion channels communicate with one another. Cryo-EM, electrophysiology, protein biophysics. A great opportunity for structural biologists to learn electrophysiology methods or vice versa. vanpetegemlab.com/opportunities.…
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Brannigan Lab
Brannigan Lab@BranniganLab·
Celebrating another year of successful collaboration with the @WChengLab at the Dandelion. Looking forward to seeing you in LA for #BPS2025!
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Grace Brannigan
Grace Brannigan@GraceBrannigan·
These simulations of membrane proteins in nanodiscs were so fun. Thank you @WChengLab for your continued enthusiasm for simulations that go beyond the basics - we are lucky computational collaborators! @VikramDalal_37 @PageAnesthesia
Wayland Cheng Lab@WChengLab

Our study on nanodiscs and ion channel structure is now published: rdcu.be/duXkK. We hope this will spur more consideration into the impact of nanodiscs on membrane protein structure. @VikramDalal_37 @PageAnesthesia @GraceBrannigan @WUSTL_AnesRsrch

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Chakrapani_Lab
Chakrapani_Lab@Chakrapani_lab·
Structural Basis for Partial Agonism in 5-HT3ARs. Tremendous effort from @KevinCFelt Maddy Stauffer, Leslie Salas-Estrada and a great collaboration with @martafilizola Pete Guzzo and Dejian Xie nature.com/articles/s4159…
Kevin Christopher Felt@KevinCFelt

Delighted to share @Chakrapani_lab's recent work on 5-HT3Rs! Here we explored the functional landscape of the 5-HT3AR to understand how orthosteric ligands with similar chemical scaffolds exhibit a range of efficacies... #CWRU #CWRUSOM rdcu.be/du8U3

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Wayland Cheng Lab
Wayland Cheng Lab@WChengLab·
@danielmihaylov7 @MishaKudryashev Also, there is still some variability in M4 resolution of ELIC structures from other studies using MSP1E3D1 and SMA. See agonist-bound ELIC in MSP1E3D1 (emd-20968).
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Daniel Mihaylov
Daniel Mihaylov@danielmihaylov7·
@WChengLab @MishaKudryashev M4 is not resolved well only in these studies, all other ELIC structures in discs have a well resolved M4. Did you try running your MD on the other structures and compare?
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Misha Kudryashev 🔬 ❄🕊️
Misha Kudryashev 🔬 ❄🕊️@MishaKudryashev·
This is a very interesting paper showing that nanodiscs, typically used to preserve lipids for structural analysis of membrane proteins, can affect the structure of the target protein. First paper I read this year, happy 2024! nature.com/articles/s4146…
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Wayland Cheng Lab
Wayland Cheng Lab@WChengLab·
@danielmihaylov7 @MishaKudryashev Yes, M4 is generally not as well resolved but there is density in the unsharpened maps to observe major differences in helix orientation. From the MD simulations, one can also see how the nanodisc can impact the orientation of M4.
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Daniel Mihaylov
Daniel Mihaylov@danielmihaylov7·
@MishaKudryashev Well that is true if the density for the helix was really there, for some cases it is rather ambiguous where the Ca should go. High restraints in phenix of courses will build anything so I would be careful when interpreting such differences. My 2cents
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Daniel Mihaylov
Daniel Mihaylov@danielmihaylov7·
@MishaKudryashev It's very interesting how they report differences smaller than the resolution reported between the structures. Building different rotamers isn't the same as having a difference but maybe people don't care any more.
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