qbase+

@Erinc2537 Hi Eric, just send a short mail to info@qbaseplus.com and we'll send you the address.

@qbaseplus Can you tell me how to get in touch with your customer service department? I need to create a PO for an order but need the correct payment remittance address. Thanks!

qbase+? That's quality control, normalization, interpretation and storage of your qPCR data. All in one application. Guaranteed worry-free! bgzlle.com/2PYhzyM

Improve the quality of standard curves by adding carrier (e.g. tRNA from brewer's yeast) to each dilution point. #qPCR #tip of the day

Deliver high quality qPCR results without losing yourself in complex formulas for normalization. Rely on our expertise that comes with a qbase+ subscription! bgzlle.com/2Q5jDp1

Not all commercially available qPCR assays have been validated in the lab; make sure you have access to (your own) validation data #qPCR #tip of the day

The median Cq value is a robust average, and obviates the need for PCR replicate outlier removal. qbase+ allows you to choose between median or arithmetic mean to calculate average Cq value. bgzlle.com/2MWUY3W

How many qPCR replicates? 1 is ok in large screening where power comes from sample size and individual errors do not affect the outcome bgzlle.com/2O7RUq6

Adhering to the MIQE guidelines improves your qPCR results and facilitates publication. bgzlle.com/2xAnzXY

Keep your budget under control by doing qPCR in 15 µl reactions (96-well) or 10 µl reactions (384-well). #qPCR #tip of the day

A final concentration of 250 nM of each #qPCR primer is a good compromise between efficiency, yield and specificity. #qPCR #tip of the day

Prefer more biological replicates over more technical replicates when trying to prove research questions. bgzlle.com/2Q09JVk

A qbase+ subscription brings return on investment: you spend less budget on qPCR repeats and less time in qPCR data normalization! Learn more at bgzlle.com/2Q09F82

If you have three genes that fit in two runs, do the measurements in three runs to avoid inter-run variation. #qPCR #tip of the day

With qbase+ default settings, even your mom can analyze your qPCR data! bgzlle.com/2xzUaNn

Use well-calibrated pipets and avoid pipeting volumes smaller than 2 µl. bgzlle.com/2Q0IHx5

Go for more simple and efficient qPCR normalization with qbase+. Learn more at bgzlle.com/2Q0DW6F

Prevalidate your reference genes for expression stability before using them in your specific experiment. More information on qPCR normalization on The Gene Expression Blog bgzlle.com/2xHcwML

Stop wasting time trying to find last year's qPCR data. Get it all organized in qbase+! bgzlle.com/2O4huMU

Measured knock-down levels depend on the sequence being evaluated. Design your assay across or near the siRNA site. #qPCR

You're welcoming a new lab member? Thanks to qbase+, transfer of your qPCR data is one thing less to think about. bgzlle.com/2xz8lCq