1/4 Our new protocol paper is out in STAR Protocols!🎉✨ We outline how to identify transcription factor (TFs) networks for immune cell reprogramming. Read full paper here: cell.com/star-protocols…

2/4 We previously developed REPROcode, a combinatorial transcription factor screening platform that works at single-cell resolution. Altman et al. now offer a comprehensive instruction manual for using REPROcode to identify transcription factor networks that drive cell identity.

3/4 The workflow includes lentiviral production, arrayed library transduction, reprogramming, cell purification, library preparation, sequencing, a downstream analysis pipeline, with quality control and troubleshooting guidance throughout.

4/4 ⭐️The framework is broadly adaptable, making it applicable across reprogramming systems and boosting accessibility to everyone aiming to seize the potential of direct reprogramming to generate therapeutic cell types.