Bukau Lab

Our first 2021 paper reveals the prevalence and mechanism of a co-translational protein assembly mechanism in human cells: co-co assembly. Congratulations to the co-co team @KaiFenzl, @matildebertolin and @guenter_kramer! science.sciencemag.org/content/371/65… @Sciart by @munafomarzia 1/6

What shall Germany deliver next? A good question posed by @faznet, thanks 🙏🏻 Well, the answer is obvious: Ukraine desperately needs 🇩🇪 fighter jets Tornados & Eurofighters💪 Dear German Government, please do not hesitate Just do the right thing! m.faz.net/aktuell/politi…

Our team will combine advanced ribosome profiling, cryo-EM and single-molecule experiments, to study how multiple ribosomes, mRNAs, nascent chains, chaperones and other factors form dynamic super-assemblies that act as coordinated protein complex assembly lines.

Great news! Together with @sjtans & @The_Ban_Lab, we are among this year's recipients of a Synergy Grant from @ERC_Research. This marks the beginning of an exciting journey into a mechanistic analysis of the biogenesis of protein complexes 🧬

Hier eine schöne Allegorie wie das von @LabJoazeiro und @PfefferCryoLab beschriebene MutS2 Protein fehlerhafte Proteinbiosynthese anhand kollidierter Ribosome erkennt und verschiedene Qualitätskontrollmechanismen initiiert bevor das fehlerhafte Protein der Zelle schaden kann.

How do cells fire up the #RQC engine? Check out our structural and mechanistic blueprint for bacterial #ribosome collision sensing by MutS2, out today in @Nature. MutS2 senses collisions and splits ribosomes to initiate RQC! nature.com/articles/s4158…

B. subtilis MutS2/RqcU deals with stalled ribosomes by binding collided disomes, recruits the RNA degradosome and initiates #RQC by #ribosome splitting. Happy we could contribute to this work led by @LabJoazeiro in collaboration with @PfefferCryoLab twitter.com/ZmbhH/status/1…

@DellaDavid_Lab @BabrahamSignal1 @BabrahamInst This is wonderful, Della. Have a great start in your new lab

I am absolutely thrilled to be relocating my lab to the Babraham Institute early next year! Looking forward to exciting collaborations with great colleagues @BabrahamSignal1, @BabrahamInst and Cambridge area.

A new study by the @bukaulab in @CellReports sheds light on role of Nt acetylation in eukaryotes cell.com/cell-reports/f…

Comparative SUMO proteome analysis identifies novel SUMO-controlled regulators in EGFR signaling - paper by a team led by @frauke_melchior embopress.org/doi/full/10.15…

@bioSavinov Hi Andrew, that's a good question! Indeed, our method may enrich for more stable interactions which survive the purification procedure. Some additional work in the lab suggests we are not missing many disomes by DiSP, but it is definitely worth further investigation :)

Latest from Bukau lab looks very cool: cotranslational complex assembly from two nascent chains is rather common! Makes me wonder what role this might play for looser, more dynamic complexes, or if the hits were enriched in tight, stable assemblies.

@AChemSynBio Thanks Abhishek, glad you enjoyed our work! That's a good question, some heteromeric subunits are indeed hits of our screening, but they await deeper investigation!!

Great reading to kick-off 2021! It turns out pairs of ribosomes, or “disomes”, coordinate translation of an mRNA such that the nascent peptide from each can come together, facilitating the formation of protein homodimers! Wonder if a similar mechanism exists for hetero-oligomers?

More details @ScienceMagazine: science.sciencemag.org/content/371/65… 6/6

Our data suggest that co-co assembly generally involves neighbouring ribosomes on one mRNA, thereby ensuring specific homomer formation of isoforms and structurally-related proteins which are highly prone to promiscuous interactions inside cells. 5/6

Our first 2021 paper reveals the prevalence and mechanism of a co-translational protein assembly mechanism in human cells: co-co assembly. Congratulations to the co-co team @KaiFenzl, @matildebertolin and @guenter_kramer! science.sciencemag.org/content/371/65… @Sciart by @munafomarzia 1/6

@Marius_Lemberg @matildebertolin @KaiFenzl @ZmbhH Thanks Marius!! Happy new year 🥳

Congratulations to our colleagues @ZmbhH for the great work!!

PNAS selected our joint work with @BukauLab on the mechanism of fiber disaggregation to be highlighted on their journal club blog! @PNASNews blog.pnas.org/2020/11/insigh…