Kelly Rios

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec. We explore 4 intracellular and intercellular applications of TransitID: - discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix) - mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress - discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery - analysis of proteins that traffic intercellularly bw tumor cells & macrophages Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor authors.elsevier.com/a/1hKLcL7PXmcqA

@BiljonRiette @Painter_Lab “Malairy Christmas” 😂 you guys are too fun

Our little Holiday masterpiece for the office door competition- featuring the lab, our many lab pets and of course, the parasite stages that best represent us ❤️🎄

Of interest to #mitochondria and #plasmodium aficionados, a paper from Ian Lamb et al. @vaidyalab #PLOSONE: Mitochondrially targeted proximity biotinylation and proteomic analysis in Plasmodium falciparum dx.plos.org/10.1371/journa…

country western blot

I am so excited, next week I will be defending my PhD dissertation! I am happy to share the zoom link for the public seminar to anyone who would like to join on Tuesday Aug 23 at noon!

@LindnerLab does kidsbop cover jazz? I thought they only did the hits, like Call Me Maybe youtu.be/_bSZUcL5Hmk

@KevinHartPhD hehe different kind of bop Kev, more kylie minogue than kinetoplastids

@KellyTRios @LindnerLab They record the BOPs(biology of parasitism)?

After extensive annotation and analysis our main analysis of TrypTag, the subcellular localisation of every trypanosome parasite protein, is up on biorXiv. biorxiv.org/content/10.110…

This is particularly important for those of us who want to study transmissible gametocytes, and other means to enrich for sexual blood stage populations should be considered.

Turns out, sulfa exposure in the rodent host or the mosquito vector limits mosquito stage development in vivo! And the effects of sulfadiazine exposure are reversible, as washing out the drug allows for fertilization and ookinete development in vitro. Check it now in @mSphereJ

My first first-author research article from my PhD with @LindnerLab is now online! 🥳 journals.asm.org/doi/10.1128/ms… Us rodent malaria researchers routinely use sulfadiazine to select for sexual blood stage parasites in the host, but we asked if this drug exposure affects transmission.

Look at these AMAZING malaria cookies that our talented tech specialist, ⁦@EmilyFern5⁩ made for world #malaria day! 😮🥰 ⁦#mayoclinmicro @AmSocParasit⁩ ⁦@mayocliniclabs⁩ ⁦@BSPparasitology⁩ ⁦@Sara_Jiang⁩ ⁦@AS_Para⁩ ⁦@Parasite_Power⁩

It’s a gorg Friday to spend the whole afternoon in a dark room with no windows 🔬

Happy to complete our trilogy on #malaria #parasites @EMBOPress on #Plasmodium sporozoites. After FORMATION in 2019, MIGRATION in 2021, it now is DISINTEGRATION. Breakup of sporozoites during skin passage limits transmission | EMBO reports embopress.org/doi/full/10.15…

@MikaelaFollmer 💚

@KellyTRios Cold blooded but not cold hearted 🦎

I love doing microscopy because I love sitting in the dark like a little vampire

@atheaslip @GriffithSci @pearcecamille I loved the illustrated schematics in this paper! Really good read, nice one guys ☺️

Our review of dense granules in #toxoplasma came out today! - Big Congrats to grad students Mike Griffith @GriffithSci and Camille Pearce @pearcecamille. Check it out! 👏🎉 onlinelibrary.wiley.com/doi/abs/10.111…

Single-molecule imaging of microRNA-mediated gene silencing in cells doi.org/10.1038/s41467…