WilsonLab

Nature research paper: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity go.nature.com/49sFSzs

Hypoxia-induced ribosomal RNA modifications in the peptidyl-transferase center contribute to anaerobic growth of bacteria dlvr.it/TQFc7k

If you're a senior researcher (i.e., associate professor or more senior), and you'd consider moving to the University of Toronto to take up a Canada Impact+ Research Chair (1 million/year in funding), email me. Discretion guaranteed. ++Please share to help me spread the word!++

Large and intricate naturally occurring structures composed exclusively of RNA... ROOL! More at Molecule of the Month: pdb101.rcsb.org/motm/313

New Biochem Soc Trans review: eIF3 as a modular, central regulator of translation. Authored by @ide_nicholas and Colin Echeverría Aitken, this review integrates genetic, biochemical, and structural perspectives and highlights open mechanistic questions. doi.org/10.1042/BST202…

Slightly overdue article highlight! In PNAS, we use smFRET to reveal how polypeptide release factor 2 (RF2) undergoes a cascade of structural changes to catalyze polypeptide release during translation termination and ArfA-mediated ribosome rescue. 🧵⬇️ doi.org/10.1073/pnas.2…

@EUjamrai's “Sketching Antimicrobial Resistance” campaign is an important reminder of the power of antibiotics. Global, multi-faceted action is needed to address AMR. @CARB_X is committed to accelerating innovative projects that target the most serious, resistant bacteria.

RNA Therapeutics Hybrid Conference rnatherapeutics.org

A Method to Watch: Predicting RNA structures nature.com/articles/s4159…

AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination | Nature Methods nature.com/articles/s4159…

New online! EMProt improves structure determination from cryo-EM maps bit.ly/48PJafU

GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome. GTPBP1 promotes more stringent mRNA decoding than the bona fide delivery factor eEF1A. Congrats to Denis Susorov and collab with Pestova lab! tinyurl.com/3smdvt3v

Great collaboration with @CarpeRibosomam and @slavicaPD labs on using MPRAs to look into non-coding mutations. Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes: Cell Reports Methods cell.com/cell-reports-m…

New online! Translational activators align mRNAs at the small mitoribosomal subunit for translation initiation bit.ly/4iCO2JI

nature.com/articles/s4159… Huge congrats to our collaborative team for their success achieving "Parallel stopped-flow interrogation of diverse biological systems at the single-molecule scale" rdcu.be/eS2ps An advance in experimental throughput and sensitivity that revealed unexpected results!

I'm working on this little ribosome game. You have to build a peptide as fast as you can. Prokaryotes can incorporate 20 amino acids per second. Currently, only the tRNAs work; the amino acids are next.

Many congratulations to @DrMartyTaylor and all the co-first authors for providing a structural understanding of how mTORC2 recognizes and phosphorylates the Akt/PKB kinase, its most famous substrate. Massive amounts of work and creativity were required to make this possible.

It was a great experience to work with a wonderful team led by @ruchianand1975. 👏👏 @unescorcb @DBTIndia @rajesh_gokhale

Saskemycin, a potent and selective antimycobacterial agent targeting a unique site on the ribosome - PubMed pubmed.ncbi.nlm.nih.gov/41282059/

Japanese researchers found a new type of archaea with the smallest known genome: 238k bases. They live inside ocean-dwelling dinoflagellates as symbiotes. Their genome "encodes the barest minimum of proteins for its own replication." All other molecules are taken from the host.