Clyde
24.8K posts
@Clydeseeks
Raise a little hell! Nobody's going to help you You've just gotta stand up alone And dig in your heels and see how it feels To Raise a little Hell of your own
No problem. The HERV-K102 ddCt quantitative PCR (qPCR) method that I actually developed while in the Blood Safety Program was for the screening of donor blood, for cases involving transfusion, for cases of transplantation, and especially suitable for cases of detecting xenozoonosis (ie., emerging pathogens) in xenotransplantation It is based on the notion that HERV-K102 particle production may be an early indicator of: an infection, a tumor/cancer, foreign DNA/RNA and/or a toxin. Any of the above might actually be increased in persons who received the spike mRNA gene therapy LNPs and does not necessarily need to be detectable in the blood per se ie., spike protein. The main principle of the method is to compare the ratio of the HERV-K102 pol signal to the 18S RNA signal (the control for genomic equivalents) of isolated DNA (the QIAmp UltraSens Virus particle Isolation kit that also copurifies genomic DNA from plasma). The method utilizes a reference genome (male genomic DNA of a known concentation) to enable quantification. Here is the reference. Laderoute MP, Giulivi A, Larocque L, et al. The replicative activity of human endogenous retrovirus K102 (HERV-K102) with HIV viremia. AIDS. 2007 Nov 30;21(18):2417-24. Generally when there is an active infection the ddCt often indicates about 10 (12) HERV-K102 particles per ml of plasma or a ddCt of 10 (9). When performed on 30 normal healthy controls validated not to have any HERV-K102 particles and no antibody to HERV-K102 envelope, the ddCt was 0.88+/- 0.37. The induction of HERV-K102 particles can be very quick such as zero particles per ml of plasma to 2.55 X 10(11) cDNA containing particles in 84 hours. My method uses an internal sequence to validate the proper sequence has been amplified (after 35 cycles), and to provide real time assessment during each cycle. My method seems to be very accurate as discussed elsewhere: Laderoute MP. Clues to finding correlates of risk/protection for HIV-1 vaccines [version 2; peer review: 2 approved with reservations] F1000 Research. 2018, 6:868. doi.org/10.12688/f1000…. If the test is performed with Amperase-UNG(TM) in the buffer, this dissolves the cDNA in HERV-K102 particles (it is a foamy retrovirus and foamy non-pathogenic retroviruses have cDNA genomes due to their opposite lifecycle to pathogenic retroviruses). Recall that cDNA contains uracil not thymine. So when 2 qPCRS are done, one with the Amp-UNG and the other not, this allows one to be confident that one has indeed measured the cDNA of HERV-K102 particles (because the cDNA signal of the HERV-K102 in the particles disappeasrs in the presence of Amp-UNG) . This method can also be used to quantitate integration levels of HERV-K102 which may indicate a recent infection/toxin. For example, a special cohort of women known to be resistant to HIV-1 acquisition for at least the past 3 years, scored positive for elevated integration (average was 5 fold over normal) which was not found in HIV-1 patients. Laderoute MP, Larocque LJ, Giulivi A, Diaz-Mitoma F. Further evidence that human endogenous retrovirus K102 is a replication competent foamy virus that may antagonize HIV-1 replication. Open AIDS J. 2015 Dec 7;9:112-22. doi: 10.2174/1874613601509010112. Others have found that increased integration of HERV-K102 in lung adenocarcinomas relates to higher survival of the host (all-cause mortality). [Ng KW, Boumelha J, Enfield KSS, et al. Antibodies against endogenous retroviruses promote lung cancer immunotherapy. Nature. 2023 Apr;616(7957):563-573. doi: 10.1038/s41586-023-05771-9. ]. Preliminary evidence suggests even small exposures to pesticides might be detected with this method in the range of 2.5 to say 5.0. The beta testing was never done despite the patents as the Director (a Hematologist) left PHAC suddenly and the new Director had no interest in blood safety and could not see the significance. So clearly the testing needs to be properly evaluated for approval by the FDA.
Et ce n'est que la pointe de l'iceberg. Demain, on publie le document complet. Et ce que vous allez découvrir est encore plus troublant. Le gouvernement Carney a annoncé le projet de Grays Bay au Nunavut, une route vers une mine d'Izok Lake. Mais à qui appartient cette mine? Au gouvernement chinois. 750 millions de vos impôts vont servir à construire l'infrastructure qui permet à une entreprise d'État chinoise d'extraire nos ressources stratégiques de l'Arctique canadien. Les Canadiens paient la route. La Chine prend le minerai. Merci à Benoît Dutrizac et à toute l'équipe de QUB Radio pour la plateforme. Le document complet sort demain. Restez avec nous.
Yet somehow not a single Ottawa Press Gallery member knew his. The media deserves to die.
The 2026 Liberal National Convention is a week away, and we’re so excited to have Diana Fox Carney join us there. If you haven’t yet, register now: 2026.liberal.ca/register. We can’t wait to see you next weekend in Montréal!
This Trump post is elite. And you’ll start to see it too as you read along. You will love this breakdown. We all know about the impending deadline on Tuesday. The deadline creates urgency for Iran and gives Trump an extra negotiation chip that didn’t exist before. We all know this. But it gets better now. Trump is now branding the deadline. “Power Plant Day and Bridge Day, all wrapped up in one, in Iran.” This branding is elite framing, as it turns an abstract military pressure into vivid, memorable visual events. People don’t remember vague threats. They remember branded days—like “D-Day” or “Shock and Awe.” Trump makes the destruction feel scheduled, inevitable, and almost celebratory. It sticks in the mind and signals total control. And notice how nonchalant it sounds. Trump didn’t go for epic, carnage-heavy branding. He branded it the way you’d casually announce National Potato Chip Day. That’s intentional. He’s making an apocalyptic-like event for Iran feel routine, even mundane, for the US. This makes the threat land harder because it flexes confidence and might. This is light work for America. Then Trump uses a direct threat. Zero diplomatic filter. It bypasses the usual State Department word salad and hits the human survival instinct. And the closer? “Praise be to Allah.” Oh. My. Goodness. He doesn’t just threaten their infrastructure- he mocks their worldview by hijacking their own religious phrasing right before promising devastation. This is too perfect. It’s a reminder who’s writing the script for their “impending” doom. This is calibrated dominance. Watch how the media spins it tomorrow. They’ll call it “unpresidential.” The people who get persuasion will see genius. What a time to be alive.
‘They used to think big’: transport minister slams Conservatives’ criticism of high-speed rail ctvnews.ca/politics/artic…
Less Than Half Of Health Care Workers Received An Updated COVID-19 Vaccine: CDC zerohedge.com/medical/less-h…
CANADA PENSION PLAN - Carney de-risking ESG Carbon Credit companies to use Canadian Pension Plan for 100 Billion India Infrastructure investment? We are never seeing that money again. The pensioners will be put on UBI using Bill S-206 - 12 Million Canadians will be forced into the new 'Stablecoin' announced YESTERDAY!
