Lehti Lab
258 posts
@LehtiLab
@karolinskainst @MTC_KI and @NTNU In Lehti group we study #TumorMicroenvironment #CancerInvasion #Metastasis #DrugResistance using 3D culture🔬🧫
The mechanical anisotropy of adipose tissues regulates ovarian cancer invasion biorxiv.org/cgi/content/sh… #biorxiv_bioeng
CONGRATULATIONS to Viviam Bermúdez and Marco Fariñas both @NTNU, and to @malvina_marku for well deserved prizes for best posters @PerMedCoE summer school! @mtrkuiper @eirini_tsi @LehtiLab
TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec. We explore 4 intracellular and intercellular applications of TransitID: - discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix) - mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress - discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery - analysis of proteins that traffic intercellularly bw tumor cells & macrophages Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor authors.elsevier.com/a/1hKLcL7PXmcqA
