Javier

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Javier

Javier

@1avier

ThinkDifferent!

Madrid, Spain Katılım Haziran 2012
155 Takip Edilen113 Takipçiler
Javier
Javier@1avier·
@JudgeTheZipper Lo hiciste gracias a tu esfuerzo y a una educación pública y de calidad
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Judge the Zipper
Judge the Zipper@JudgeTheZipper·
Hijo de ama de casa y funcionario de grupo B (único ingreso en casa). Cuatro hermanos. Mi madre: estudiad lo que queráis el tiempo que necesitéis, que aquí se ponen las mismas lavadoras y la mismas lentejas para todos. Ahora soy juez y resulta que mis padres eran ricos. Y yo que pensaba que me querían y que su prioridad era nuestra educación.
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Javier
Javier@1avier·
@JudgeTheZipper Lo hiciste gracias a tu esfuerzo y a una educación pública de calidad.
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Mónica García
Mónica García@Monica_Garcia_G·
¿Ya sabéis con quién vais a ir? ☺️
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National Academy of Sciences
National Academy of Sciences@theNASciences·
We’re pleased to announce the 2023 NAS Awards recipients! These 16 individuals are honored for their extraordinary scientific achievements in a wide range of fields spanning the physical, biological, and medical sciences. bit.ly/402ovjs #NASaward #Science #Awards
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José Carrillo
José Carrillo@pepcarrillo·
1) En pocos días la UCM nombrará a Isabel Díaz Ayuso “alumna ilustre”. He evitado hacer pública mi postura sobre dicho nombramiento con la esperanza de que no se llevaría a cabo. Hoy, pérdida toda expectativa, hago públicas las razones por las que discrepo de ese nombramiento.
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Javier
Javier@1avier·
@delarosatrevin Enhorabuena Josemi! Todo lo mejor para tu nueva aventura americana ☺️
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JM de la Rosa Trevin
JM de la Rosa Trevin@delarosatrevin·
I haven't tweeted in a while but...a new stage in my life begins and it might deserve a tweet. For many reasons we decided to leave the comfort in Swedish and start the American adventure. Really excited to join Pirate Fernandez and his crew at the CryoEM facility in St Jude!!!
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Javier
Javier@1avier·
@IsraelF96135088 DeepEMHancer trains on a large set of different maps and LocScaled maps. I don't see why two specific DE and LS maps should be identical. Also, DeepEMhancer performs masking based on PDB maps. So DeepEMhancer = local scaling + tight masking
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Israel Fernandez
Israel Fernandez@IsraelF96135088·
I have two questions: -why deepEMhacer and LocScale maps looks different? deepEMhacer is trained on LocScale generated maps no? Why they are different? -deepEMhacer map "looks" better, with RNA base-separation quality in a region with ~4Å resolution. How is this possible?
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Israel Fernandez
Israel Fernandez@IsraelF96135088·
OK, sequel of the sharpening drama, this time a bit more interesting. Same map as yesterday (~2.2A bacterial ribosome with 2xtRNAs) but this time focused on the small subunit (30S) head, which is always flexible. Unsharpened map colored according to local resolution estimation 👇
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Javier
Javier@1avier·
@CryoTUD @SjorsScheres @biochem_fan I would have to take a look to the code to refresh but I think the normalization is based on percentil values obtained from the maps ( (x-p5)/(p95-p5)) followed by a clipping process. This will compress the histogram
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とりさん
とりさん@biochem_fan·
I am not OK with refinement against deepEMhancer-ed maps but apparently many people think it is OK. Fine. Maybe I am too old-fashioned.
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Javier
Javier@1avier·
@SjorsScheres @biochem_fan @IUCrJ I have not read the paper in detail but I think authors claim to use the previous knowledge accumulated in deepEMhancer to improve the alignment and seems to work. I understand that the resolution is obtained from Relion maps
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Javier
Javier@1avier·
@CrollTristan Those low countour "features" (why not calling it noise?) are a consequence of chunking the map into cubes, and their intensities should be negligible compared to the rest of the volume. What is the contour level?
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Tristan Croll
Tristan Croll@CrollTristan·
Can any #cryoEM folk tell me what on earth is going on in the low contours of this map (EMD-32679)?
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Javier
Javier@1avier·
@SjorsScheres @biochem_fan DeepEMhancer maps are not supposed to be used to evaluate resolution values. I think this is explicitly said in the DeepEMhancer paper. Agree that validation is key in cryoEM
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Javier
Javier@1avier·
@Elena_Salamanca Políticos tenemos muchos, Periodistas parece que no tantos. Usted debería ser (o al menos parecer) imparcial.
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Elena Salamanca
Elena Salamanca@Elena_Salamanca·
Este es el tipo de gente a la que moviliza Pablo Iglesias cuando señala a un "asalariado", un trabajador/a, de una empresa que a él no le gusta. Más de 2.300 tuits en 11 horas.
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