Joshua Blodgett

231 posts

Joshua Blodgett banner
Joshua Blodgett

Joshua Blodgett

@ActinoLab

Biology faculty at Washington University in St Louis. Microbiology, biosynthesis and biotechnology!

Katılım Ocak 2018
98 Takip Edilen230 Takipçiler
Rothmus 🏴
Rothmus 🏴@Rothmus·
BBQ map. Don’t @ me.
Rothmus 🏴 tweet media
English
1.3K
508
8.1K
643K
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@drnaomi I've seen a hoarder house first hand (and the fallout after the fire department needed to respond). The one I saw was nothing but old newspapers, magazines and actual trash. Hoarding has serious mental illness implications, rich or poor.
English
0
0
0
9
Joshua Blodgett retweetledi
Kalindi Morgan
Kalindi Morgan@kalindi_morgan·
Piperazate-Guided Isolation of Caveamides A and B, Cyclohexenylalanine-Containing Nonribosomal Peptides from a Cave Actinomycete | Organic Letters pubs.acs.org/doi/abs/10.102…
English
0
1
8
295
Joshua Blodgett
Joshua Blodgett@ActinoLab·
Polycyclic tetramate macrolactam genes are common in Streptomyces. But don't confuse that with boring. Check out our recent work if you are interested in orphan genes, BGC mosaicism, and lingering challenges in translating genes to molecules journals.asm.org/doi/full/10.11…
English
0
0
1
75
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@wildtypeMC You might have to reach out to the chair of Stanford Biochem to track the disposition of Dale's strain collection.
English
0
0
1
88
Marc Chevrette
Marc Chevrette@wildtypeMC·
Does anyone know where to find Myxococcus xanthus DK1622? I'm not able to see it in online strain collections.
English
5
2
2
2K
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@ATinyGreenCell Actinomycete PCR is challenged by high G+C DNA. Our colony screens are done using cells ground in DMSO (adapted: doi: 10.1016/s0167-7012(02)00235-x.. 2003 Jun;53(3):401-3.J Microbiol Methods). Then PCR is done using FailSafe Premixed buffers.
English
0
0
0
30
Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
DMSO is my lord and savior. Ladder Genomic Amp Genomic Amp Genomic Amp + DMSO Genomic Amp + DMSO Genomic Control It worked! Kinda too well and got some secondary amps. No issue I'll just cut that out during digest. Purifying now!
Sebastian S. Cocioba🪄🌷 tweet media
Sebastian S. Cocioba🪄🌷@ATinyGreenCell

Alright, giving this citT citrate antiporter gene another Q5 PCR attempt. This time testing with and without DMSO (10% v/v final). If this doesn't work then I'll just do nested PCR. I'm getting this gene out come hell or high water, lol.

English
5
5
94
12.6K
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@wwwplumcom Like others have said, this is most likely an actinomycete. To obtain even growth, use a sterile, low volume mortar and pestle and grind to an even suspension- then save directly as a cryostock or use to sub culture. We do this all the time with environmental isolates.
English
0
0
2
441
allis🦠n ✨🇺🇸➡️🇯🇵
#microbiology Twitter, any ideas what to google for this phenomenon? when bacteria form hard-to-break-apart balls in the bottom of liquid cultures? I think I'm going nuts.
allis🦠n ✨🇺🇸➡️🇯🇵 tweet mediaallis🦠n ✨🇺🇸➡️🇯🇵 tweet mediaallis🦠n ✨🇺🇸➡️🇯🇵 tweet mediaallis🦠n ✨🇺🇸➡️🇯🇵 tweet media
English
22
16
128
37.5K
Joshua Blodgett
Joshua Blodgett@ActinoLab·
Kudos to undergraduate @ActinoLab member @grace_dekoker for publishing her public-facing summary of pnas.org/doi/10.1073/pn… on @sciencebreaker @WUSTLBio #SciComm #OpenScience
TheScienceBreaker@sciencebreaker

Ever wondered where drugs come from? 🤔 Discover how rare soil #bacteria could hold the key to new treatments for diseases like #cancer! 🔬 Our latest article unveils a promising new molecule with unique properties. #scicomm #openscience ow.ly/CMlc50NoI4i

English
0
3
6
810
Zack Buchan
Zack Buchan@zackbuc1·
I gotta say I seemed to see way fewer people wearing Purdue gear this weekend while out and about in Indy, not sure what that was all about?
English
2
0
7
874
Brett Phinney
Brett Phinney@UCDProteomics·
Well I finally got my first geeky mass spec tattoo ! At age 51 no less ! And now I get to explain it to everyone who asks what the heck it is !
Brett Phinney tweet media
Davis, CA 🇺🇸 English
44
36
923
107.2K
Joshua Blodgett retweetledi
WashU Biology
WashU Biology@WashUBio·
We are thrilled to announce that Assistant Professor Josh Blodgett will be promoted to associate professor in July! Congratulations @ActinoLab!
WashU Biology tweet media
English
3
2
10
630
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@BiologyCarly Undergrad story...Doing dozens of West Nile Virus Crow necropsies when my fellow tech cuts straight through a hemorrhaged skull and into her finger with bone shears. With no cure, it was "go wash up and let us know how you feel...". It got real quiet from there.
English
0
0
6
259
Carly Anne York, Ph.D.
Carly Anne York, Ph.D.@BiologyCarly·
What is your most unhinged story from grad school? Mine was probably the time I picked a dead flounder up off the lab floor and then ran to a public bathroom with it in my hands and sobbed over its body in a stall.
English
204
130
1.7K
2.1M
Joshua Blodgett
Joshua Blodgett@ActinoLab·
@academic_exit I went from post doc to industry then back to academia. While I think I know what you are saying, I’m unsure it’s quite that simple for everyone everywhere.
English
0
0
1
348
Academic Exit
Academic Exit@academic_exit·
When I moved from my post-doc to my first industry role, my base salary went up by more than 300%. Do you love your academic topic enough to under-earn that much? Or want to earn enough that you don't have to think about money every week at the grocery store? Your choice.
English
65
114
1.8K
427.8K