Nolan Amon

My first, first author paper got published :) cambridge.org/core/journals/…

Hello Science Twitter! I do still exist, and am doing lots of fun metagenomics things. On that note, I was wondering how I might be able to retrieve the entire taxonomic hierarchy for a list (~2000) of organisms. There seems to be an R package (taxize) but it seems broken :(

(why yes I did just get my first illumina seqs back and now I'm messing around like a toddler who got into power tools, why do you ask?)

Hey science Twitter, it's been awhile!! So just wondering, what are everyone's favorite programs for classification in shotgun metagenomics and if you're using kraken2 what is your favorite database and confidence score to use? 👉👈

I've been fiddling with some R code for three days and it FINALLY is working, there's almost nothing more gratifying.

My son, the bee

I was chosen 😤

Trying to figure out coverage based rarefaction with #iNext in R- lots of examples given where you are comparing two groups/treatments with a single collection event, but I can't wrap my brain around how to handle data sourced from multiple collection events! Any advice?

At the barber today and he asked me if I go to school in Madison. I said yes, and then he followed up by asking what grade I was in- as he was cutting my head full of graying, Ph.D. related hair 😅

@Jenna_M_Walters I agree with Bombus- I think this is a very weird, very sad impatiens!

Bee/taxonomy folks! Got this lady from a friend in Brighton, MI. The legs look crazy long and super short/stout abdomen. Any ideas on ID?

Please tag any aquatic ecologists who might know!

Culturing some pond water to try getting live food for my fish, does anyone know what these y-shaped/tri-lobed little guys are?

It took me literally all day and about 100 different browser tabs but I analyzed my first non-tutorial microbiome dataset :)

@tahneeames I'll let you know once the samples come back ;)

@Anthophilist Oooooo how do you find RNAlater? I’d never used it but I’m considering it as an option for remote collecting (seems easier than shipping live insects)

Samples dropped off this morning! Can we get good enough quality and quantity for metatrancriptomics? I'm doubtful but time will tell!

@tahneeames The samples are in RNAlater! But our biocore set out a pail of wet ice for sample submission, even RNA 🤷‍♂️

@Anthophilist Whoa do you store on wet ice?

Running an ordination on some community data- I don't know what Procrustes means and at this point I'm too afraid to ask.

How would one hypothetically begin to organize a general strike

For reference, community is relatively species rich (119 species) but not very even- almost a quarter of those 119 species are represented from only 1 or 2 collection events. Strong bias towards halictids, as might be expected from pan traps.

Hello nerds! Working on stats for my master's research examining effects of wildflower plantings on wild bee communities in cranberry. Which biodiversity metrics/indices do you think should be reported in a manuscript? (1/2)