Benjamin Grodner

Our paper! 🪩 "Spatial mapping of mobile genetic elements and their bacterial hosts in complex microbiomes" Full text link: doi.org/10.1038/s41564…

@ShaunTCross @IwijnDeVlaminck Thanks Shaun!!

Congrats @BenjaminGrodner and @IwijnDeVlaminck! If you want to see some really cool multiplexed FISH (DNA and RNA) imaging for microbiome studies take a read! nature.com/articles/s4156…

Check out the manuscript for all the lovely details and discussion! doi.org/10.1038/s41564…

This method can help advance the study of microbial ecology, serving as a complement to metagenomic sequencing by revealing the spatial interplay between bacteria, plasmids, and phage.

Our paper! 🪩 "Spatial mapping of mobile genetic elements and their bacterial hosts in complex microbiomes" Full text link: doi.org/10.1038/s41564…

📢 Short talks: Dr Ben Grodner @Cornell ✅HiPR-FISH (PMID: 33268897) maps genera spatial heterogeneity (#biofilms) ✅MGE-FISH mapping mobile genes ✅overlap tools to determine interactions and spatial ecology doi.org/10.1101/2023.0… #HavingIMPACTT2023 @IMPACTT_Canada

Elegant technique from @BenjaminGrodner using molecular barcoding to visualize mobile DNA transfer - many applications to microbiome studies, bacterial genetics, and basic biofilm microbiology. Also - beautiful images. #HavingIMPACTT2023 @IMPACTT_Canada biorxiv.org/content/10.110…

First, the methods could be employed to investigate the processes that govern the emergence of antibiotic resistance. Second, MGE mapping can help address the challenge of determining bacteriophage host taxa, which is crucial in phage therapy.

We suggest that MGE mapping can serve as a direct complement for metagenomic sequencing of spatially structured microbiomes. We envision two potential application areas.

I’m very pleased to share our new preprint where we simultaneously map MGEs and bacterial taxa in complex microbiomes, revealing their spatial structures.