@EmbaixadaEUA @grok, isso nao eh interferencia na politica externa?
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Rafael Lira
174 posts
Rafael Lira
@BiophysLira
🇧🇷 - 🇩🇪 - 🇳🇱 Biophysicist and music enthusiast
Katılım Mart 2021
721 Takip Edilen300 Takipçiler
Dia 7 de setembro marcou o 203º Dia da Independência do Brasil. Foi um lembrete do nosso compromisso de apoiar o povo brasileiro que busca preservar os valores de liberdade e justiça. Para o ministro Alexandre de Moraes e os indivíduos cujos abusos de autoridade têm minado essas liberdades fundamentais – continuaremos a tomar as medidas cabíveis.
Under Secretary of State Sarah B. Rogers@UnderSecPD
Yesterday marked Brazil’s 203rd Independence Day. It was a reminder of our commitment to stand with the people of Brazil who seek to preserve the values of freedom and justice. For Justice Alexandre de Moraes and the individuals whose abuses of authority have undermined these fundamental freedoms - we will continue to take appropriate action.
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By following GUV growth and bud size in real-time, it is possible to determine the changes in reduced volume (v) and spontaneous curvature (m) and locate the growing vesicles in the shape diagram, with flip flop resulting in a milder increase in m for a given v.
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The addition of CerC6, a molecule that quickly undergoes flip flop, relaxes curvature stresses and lead to the generation of much wider buds
GIF
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The reconstitution of growth and division in synthetic cells faces many challenges. Here, we show one of the most efficient growth system ever described, followed by division, with lipid flip flop as a key regulator of membrane shape
biorxiv.org/content/10.110…
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The work was done at @tudelft under the excellent supervision of @cees_dekker
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Fusion of small liposomes to GUV model membranes result in a substantial increase in GUV area. Growth is, nonetheless, accompanied by budding due to curvature effects
GIF
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The buds do not share aqueous connectivity with the main GUV membrane. Photobleaching of an water-soluble probe in the GUV lumen does not result in exchange with the bud content
GIF
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Fusogenic liposomes are an excellent alternative to internalization-based particles for intracellular delivery of cargos. But they don't come without effects.
Here we show how
onlinelibrary.wiley.com/doi/full/10.10…
@univgroningen, @FLIM, @GUVs, @drugdelivery
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@js_neuro It's possible he/she didn't even realize you needed to be acknowledged. Did you try to talk to them? Something similar once happened to me during the proofs (my former boss and I had a great relationship) and I joined the final version of the paper
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Looking for recommendation #AcademicTwitter
What do you do if your ex-supervisor publishes work you contributed heavily to without giving you authorship? Looking for specific action points!
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O único problema deste vídeo é não mostrar o debate até o fim.
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QME
Polymer droplets exhibit a set of material properties. Small polymers require high concentrations for phase separation. Ultralarge polymers achieve it at much lower concentrations. Are their properties characterized by polymer size, concentration, or both?
Here we address this:
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@BiophysLira Presumably yes.
Fluorescent dextrans were encapulated inside of a mother, and daughters also showed the fluorescence.
If multilamellar, outermost bilayer would grow and be peeled off, and the fluoresence encapsulated by innermost bilayer would not be distribute in daughters.
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I made an amazing discovery!!!
It's a cascade division mechanism of lipid vesicles, which I named "Osmotic Spawning".
A single mother vesicle spontaneously produces 30-300 daughter vesicles.
Check out my first corresponding author paper!
pubs.rsc.org/en/content/art…
(1/2)
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@ja_steinkuehler @Kiel_Nano @JPhysChem Hi Jan, super cool and a hell of a team!
I wonder if there's experimental data on this, from you or anything similar that you may be aware of. Congrats
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A little insight on synthetic cell division: Specific peptide sequences have the potential to induce sufficient membrane curvature for full vesicle division when adsorbed to the inner membrane leaflet. Read our publication pubs.acs.org/doi/10.1021/ac… @Kiel_Nano @JPhysChem #synbio
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@LeventalLab Thanks, Ilya. There will be more stuff coming in this direction - hopefully soon - that may seem not so unfamiliar to you =)
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oh this looks interesting and important...
Rafael Lira@BiophysLira
Membrane fusion-dependent cargo delivery results in the incorporation of large amounts of foreign lipids in the cells' PM. Using FLIM, we show that lipid incorporation fluidizes the PM and reduces surface tension, leading to internalization of the excess of lipids
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Liposomal drug-delivery vehicles that fuse with cells and directly and quickly deliver their cargos in the cytosol can be extremely efficient, but this efficiency may come with a price. Here we show how.
biorxiv.org/content/10.110…
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