CRISPRGuru

I received these award-plaques recently, in a ceremony at my institute, for innovating a better CRISPR method called Easi-CRISPR. See link for a short story of how it came to be.... linkedin.com/feed/update/ur…

@mmahesh1 @seaportboston @VisitBostonCity Saada dose and kobari chutney here

Boston Diaries: “Dosa is the Answer” said the background @madrasdosacompany near @seaportboston in @VisitBostonCity Earlier I didn’t had the right question, now I had Question: “Am I hungry for the dosa?” Answer: Yes, I am hungry, I better had the dosa! So I did!

@HarmitMalik SKY's catch was absolutely terrific. It could be one of the greatest catches in cricket history, if not the greatest imo.

Two amazing overs from Bumrah and some amazing support from Arshdeep and Hardik Pandya (not to mention that unbelievable catch under pressure by Suryakumar Yadav at the boundary line) alas South Africa stumbled again from a winning position. Check out that win probability graph!

@HarmitMalik What a roller coaster game!

Scientists working in core facilities make a huge impact to research output. @theNCI's R50 funding opportunity supports core scientists. Do you want to learn more about R50? Join today's @MyABRF Town Hall zoom meeting at this link, 2pm EST. abrf.org/event/abrf-vir…

@KrishnanYamuna This chap may also build chaperones, as evolution progresses.

I am excited to be speaking about novel CRISPR genome engineering methods at Discovery Europe 2024 conference, May 22-23, Basel, Switzerland. oxfordglobal.com/discovery/even…

Sunday Brunch: Jowar Roti (ಜೋಳದ ರೊಟ್ಟಿ) and Stuffed brinjal/Badanekayi Ennegayi (ಬದನೆಕಾಯಿ ಎಣ್ಣೆಗಾಯಿ)

The Sky is blue and the Sun is bright now in Nebraska, before the eclipse. It will be partial here though!

Looking forward to speaking about "Emerging Technologies in Genome Editing" in two weeks at the ABRF 2024 meeting.

Sunday morning breakfast: Rava Idli (ರವೆ ಇಡ್ಲಿ) and Coconut-Peanut Chutney (ತೆಂಗಿನಕಾಯಿ-ಶೇಂಗಾ ಚಟ್ನಿ), all made from scratch.

Sunday morning breakfast, ಮಸಾಲೆ ದೋಸೆ!

This new (simplified) method is a new addition to other short- and long- range sequencing based approaches, like CRISPR-KRISPR and CRISPR-LRS ncbi.nlm.nih.gov/pmc/articles/P… genomebiology.biomedcentral.com/articles/10.11… x.com/CRISPRGuru/sta….

A simplified method to find out if DNA inserted correctly in your CRISPR mouse 3 Steps: Isolate genomic DNA, enrich target region via Cas9 (cut & ligate adapters) & long range sequence. Shown here for AAV CRISPR KI mice: can be used for any CRISPRed mice tinyurl.com/4ct5y93v

@mmahesh1 @Bhoothayi I guess, your guess is correct!

@Bhoothayi I suppose it is from “Guru Sweets” Photos taken during my recent visit

ಗೂಡು ಮೈಸೂರುಪಾಕ್ from ಪೆಟ್ಟಿ ಅಂಗಡಿ is always yum ❤️😋

CRISPR guide RNAs & DNA primers are widely used reagents in biomedical research. Are these reagents always pure, without any contaminants? Probably not! Here is our analyses of reagents procured from several major suppliers in Europe, Asia and USA. shorturl.at/ovUW1

A new wave of CRISPR-delivery technologies? See link for info linkedin.com/feed/update/ur… tinyurl.com/mpu5nvsu tinyurl.com/kcx4r7um tinyurl.com/swznneew tinyurl.com/mt5r7w2v tinyurl.com/3b7hvnsh tinyurl.com/49dwcbzz tinyurl.com/46xaawk9 tinyurl.com/2s3vd2rd

If scientists get ‘curious’ and follow-up why some of their experiments showed weird results—instead of thinking their experiments did not work—is when new unexpected (but potentially breakthrough) discoveries are made. You will see such an example in this tweetorial!

This is an 'exceptional technological advance' in turning on/off of gene expression at mRNA levels. Very impressive! nature.com/articles/s4158…

@mmahesh1 That is right.... life was absolutely very simple those days. Just two or three pairs of clothes per year. I brought two lungis that my father used to wear before he passed away in 2020 and I wear them occasionally in summer here in the USA, to remember my college days!

Back to Basics: This was how I used to dress in traditionally when I went to college in Mysoru - also as my father was retired, kept my expenses low (#ALARA) by being a ‘minimalist’. Owned only two pants (didn’t have to waste time in selecting what to wear) Life was simple!

@mmahesh1 You guessed it right 😀 I saved it only for myself but I shared the other condiments with others!

@CRISPRGuru I bet you didn't share it with others - because I would do the same!

Last working day of 2023-got lot done Wanted to make "Maddur Vade (ಮದ್ದೂರು ವಡೆ) I remembered while going from Mysore to Bangalore on train, as soon as train stops at Maddur - we all used to run to buy Maddur Vade. Even though it is not the same but not bad for 1st try.