Charlotte Crowe

Mechanistic and structural insights into degrader-targeted protein ubiquitinability, now out in @ScienceAdvances! science.org/doi/10.1126/sc… Let's make more degraders that hit the bull's eye 🎯 @alessiociulli @UoDCeTPD

An absolute pleasure to work with all co-authors on this study: from @alessiociulli’s lab (the 🤝 House of VHL 🤝) @ales_salerno & Gajanan Sathe – and our wonderful collaboration Graham Marsh and Hannah Maple from @Tocris @biotechne.

We combined cryo-EM, chemical design & synthesis, ternary complex and ubiquitination biophysics & biochemistry, and cellular profiling to tease out the mechanisms at play.

Tuning linker rigidity and length determines whether a ternary complex orientation leads to: - Efficient substrate ubiquitination & degradation, - Partial or inefficient ubiquitination, - Or complete stabilization through a locked, non-productive geometry.

From degradation to stabilisation! Reprogramming the VHL-degrader CM11 into VHL-stabilisers through linker design, locking a non-ubiquitinable ternary complex. 🔗biorxiv.org/content/10.110…

Excited to see how this MGPROTAC concept evolves and what new disordered targets we can take on next!

Huge well done to our co-first author Carlo Verhoef and all our fantastic co-investigators @Mark_A_Nakasone, Aitana @DeLaCuadraBaste, Tessa Harzing, Naomi Span, Gajanan Sathe, Kentaro Iso, @COttmann1433, Luc Brunsveld and - last but not least @AlessioCiulli & Peter Cossar

House of VHL meets house of 14-3-3 🤝 Presenting our study “Induced ubiquitination of the partially disordered Estrogen Receptor alpha protein via a 14-3-3-directed molecular glue-based PROTAC design” 🔗biorxiv.org/content/10.110…

After the Krios I's 10-year birthday earlier this year (full story can be found here: lnkd.in/eCKF3vBF), it was awesome to visit the eBIC facilities at @DiamondLightSou and see that the epic party hat still lives on 🥳

Awesome view over the river Tay yesterday afternoon, on the train heading down to @DiamondLightSou for the @ccp_em Icknield workshop -- the famous haar (fog) was in full force ⛅️

Catch up on the latest #TPD literature, check out the August-September edition of the @UoDCeTPD Journal Club!

@MichaelJFoxOrg Our @UoDCeTPD PIs @AlessioCiulli, @farnaby84 and Peter Cossar represented the team presenting progress on our several ongoing projects with Aina, @alex_hallatt, @_Deenwood, Jack and Zher Yin

Fantastic experience attending the LITE Annual Meeting 2025 in San Francisco, as part of the @MichaelJFoxOrg initiative to accelerate therapeutic discovery in Parkinson’s disease An exciting few days of science with academic and industry partners from across the consortium!

Had an awesome time at the 3rd Scottish Symposium on Cryo-EM! Great to see the community growing, get updates on the exciting equipment🔬 across Scotland and catch up about projects! Many thanks to the Edinburgh team @mdrwilson lab for hosting us this year 😊

Celebrated graduating last June with my favourite people! Grateful to have my family together to mark the occasion!

@nurkocaturk Exciting 🥳

Current mood: working on revisions with 2 laptops and 3 screens…

Looking forward to presenting this Thursday along with @ales_salerno! @DanaFarber @biomiko

Looking forward to participating in the @DanaFarber Targeted Protein Degradation webinar series this Thursday! Registration is open: dfci.zoom.us/webinar/regist…

The Feb-March Journal Club edition is finally here!! Thanks to our amazing editors: @justgiorgia, Angus, Gajanan and Stefan. Read to catch up with the latest literature in TPD! #openaccess #TPD #journalclub @UoDLifeSciences sites.dundee.ac.uk/alessio-ciulli…

Following on from @UoDCeTPD @CJ_Diehl's mega literature review of VHL in @ChemSocRev 2022... pubs.rsc.org/en/content/art… We now have @alex_hallatt, Aina, Jack and @alessiociulli's impressive VHL ligand patent review! Incredible team effort 👏 🔗 tandfonline.com/doi/full/10.10…