Choudhary Lab CPR

Congrats to Takeo (@TakeoNarita80) who led the work and to all the co-authors.

By revealing the scope of cohesin looping in gene regulation, and by disentangling CTCF´s looping-dependent and -independent roles of CTCF, the work offers a unified model of how Cohesin and CTCF impact gene expression.

Cohesin and CTCF jointly fold genomes, but why do their depletion affect few genes, with minimal overlap? Do all regulated genes require looping and enhancers? In our new paper in Nature Genetics we disentangle their functions in gene regulation. nature.com/articles/s4158…

Amazing work by Takeo (@TakeoNarita80) on these comprehensive analysis and many new insights.

We propose that gene-skipping by enhancers are the exception, not the rule. Linear enhancer-promoter distance and coactivator requirement of genes can predict enhancer targets with accuracy rivalling state-of-the-art experimental approaches.

Do enhancers often skip active genes? Our other recent preprint revises current models, that enhancers frequently and by design skip active genes to activate distal targets. Instead simple rules can predict enhancer and GWAS targets. biorxiv.org/content/10.110…

Great effort by Takeo (@TakeoNarita80) and the rest of the team in getting together this work.

Our findings resolve several long-standing conundrums regarding the involvement of cohesin and CTCF in gene regulation, and we present a unified model for their combined and individual roles acting directly near promoters or in concert with p300/CBP-dependent enhancers.

How do cohesin and CTCF control gene expression and what are their links to enhancers? Our new preprint unravels their shared and unique roles: from precise enhancer targeting to direct transcriptional activator functions of CTCF. #Genomics #CTCF #Cohesin biorxiv.org/content/10.110…