
Sagar
132 posts

Sagar
@CryoSagar
Fiddling with the code and instruments for CryoET in between coffee, good food. Opinions are my own.





Same for @CryoSagar and STOPGAP, which was instrumental here. But it still remains really difficult to make this kind of analysis because the processing pipeline is not as streamlined as in SPA. Actually, sometimes it really looks like that:


Our manuscript for STOPGAP is now out in @ActaCrystD! It was great working with @CryoSagar and twitterless Jonathan Wagner. journals.iucr.org/d/issues/2024/…


Hey #teamtomo Here is EMPIAR-11830! Some 1800 tomograms from awesome #Chlamy (Chlamydomonas reinhardtii) using large scale #cryoET! In addition , all the metadata and preprocessing as #minimalproject to get you started right away! #TOMOMAN #bigdata #communityscience 1/

The new @EMDB_EMPIAR cryo-electron tomography (cryo-ET) browser allows you to see proteins up close in the cellular environment. Over 1,800 high-resolution reconstructed tomograms from cryo-plasma-FIB milled Chlamydomonas reinhardtii cells are available. ebi.ac.uk/about/news/ann…

Over 4 years in the making, I am excited to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells. A 🧵



Just in time for Festivus, our preprint on STOPGAP is out! In it, we describe the subtomogram averaging algorithms we find useful. It was great working on this with @CryoSagar and twitterless Jonathan Wagner. biorxiv.org/content/10.110…









