Daniel Cetnar

This is a great paper from the @hsalis lab. - Measure the decay rates of 50,000 mRNAs in bacteria. - Use biophysical models + ML to build models of mRNA stability. - Profit. And a good reminder of what's possible when one turns a biological problem into a sequencing problem!

Designing synthetic mRNAs 🧬 for optimal stability and expression? Checkout our work led by @DanielCetnar on biophysics infused machine learning approaches for delineation of mRNA degradation kinetics ⚡ out now in @NatureComms nature.com/articles/s4146…

I am pleased to announce our latest publication ‘Predicting synthetic mRNA stability using massively parallel kinetic measurements, biophysical modeling, and machine learning’ in @NatureComms

A big thank you to @bioalgorithmist , @grace_vezeau , and @hsalis for making this work possible!

The developed model quantifies the key interactions that collectively control mRNA stability in bacterial operons and predicts how changing mRNA sequence alters mRNA stability, which is important when studying and engineering bacterial genetic systems.

NEW PAPER led by @grace_vezeau! We rationally engineered synthetic riboswitches to carry out sense & respond for human biomarker proteins [mCRP and IL32-gamma] that indicate disease or infection. 16-fold activation without trial-and-error! nature.com/articles/s4146…

Our manuscript on Promoter Calculator was published today in @NatureComms! Many thanks to @bioalgorithmist and @hsalis for helping to complete the project. Debug genetic systems and forward engineer new promoter sequences. Read more here: nature.com/articles/s4146…

If you are tired of Wordle, my friend created a new word game called Neword! Try it here: purefit.life/neword/

See our recent work at the @hsalis #synbio lab. DNA sequence in --> transcription profile out. Validated on 17396 sig70 promoters. Use it to identify cryptic promoters in genetic systems or to forward engineering synthetic promoters. Work done by myself and @bioalgorithmist!

Congratulations to Penn State chemistry's newest graduate, Dr. Joshua Kauffman! Josh, who is a member of the Sen group (@ayusmansen), recently defended his thesis.

@alexcampreis Thanks @alexcampreis! Excited to soon join you in Boston!

Congrats @DanielCetnar on a successful PhD defense! Impossible to measure the extent of your impact on the Salis lab (including my work) and the entire PSU ChE department!

NEW PAPER: We uncover & quantify how the 5' UTR, intergenic, and 3' UTR regions control mRNA decay + develop predictive biophysical models. Big 👏🙇 to @DanielCetnar who characterized 82+ operons using RT-qPCR to show clear cause-effect outcomes. pubs.acs.org/doi/10.1021/ac…

@bioalgorithmist @CodeyMathis youtu.be/gdJWZxPW45c

@CodeyMathis Muzak from the 60s is the only correct answer. @DanielCetnar knows the truth.

This Week on Friday, September 4, 2020 at 2 PM PST We are Hosting Professor, Xun Tang. Talk Title "Modeling and control for molecular self-assembly and the construction of genetic circuits" Apply at BioCompute.Club

@Xun__Tang Congratulations Xun!

Thrilled to start my first day as an Assistant Professor at LSU ChE!

ATTENTION #synbio: Design 1000s of non-repetitive genetic parts using the Non-Repetitive Parts Calculator. Ayaan Hossain @bioalgorithmist leads you through the step-by-step process for promoters, insulated RBSs, terminators, and toehold RNA switches: github.com/ayaanhossain/n…