David Breslow lab

Imaging-based CRISPR genome screening allows effects of cell-regulating molecules & modifications to be revealed in live & fixed cells – applied here to frog embryo cilia 📹 Jingbo Sun et al @DavidBreslowLab @Yale in @Dev_Cell ➡️ bpod.org.uk/archive/2026/1…

Lots of potential for future ciliary (and non-ciliary) screening applications. Great work by all authors, especially postdoc Jingbo Sun. And great collaboration with the Khokha lab!

SMIM27/TZMP1 KO cells don't form cilia. TZMP1 localizes to the transition and by IP/MS is part of the MKS complex at the transition zone. With Mustafa Khokha, we find conserved roles in ciliary function in Xenopus embryos.

Ever wanted to do a genome-scale CRISPR screen for a microscopy-based phenotype? Pooled CRISPR screens are amazing, but hard to combine with microscopy... Excited to share a technology platform for such imaging screens and applications to primary cilia... cell.com/developmental-…

Online now: A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology dlvr.it/TPNHyB

Great work by all authors, especially first Breslow lab grad student Shane Elliott. And thanks also to excellent collaborations at Yale with labs of Angelique Bordey and Tony Koleske

3) it is noteworthy that a GOF CRISPRa screen was able to uncover a connection to a disorder caused by somatic activating mutations; possibly CRISPRa screening may be broadly useful for studying such diseases caused by somatic mutations ...

Excited to share our latest work on a new cilia disassembly pathway & a link between this pathway and the neurological disorder focal cortical dysplasia: science.org/doi/10.1126/sc… Summary: From a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...

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Proud to share our work by a fantastic team, spear-headed by PhD student @Kainerox with major contributions by #xenopus expert @RosiFeistel. We developed an improved iAPEX-based #ProximityLabeling method for #massspec based subcellular #proteomics. biorxiv.org/content/10.110…

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