Elo Madissoon

Check out our lung cell atlas showing airway immune niche (among other things) using single-cell and spatial transcriptomics data.

We’re thrilled to finally share the integrated Human Lung Cell Atlas (HLCA), a single-cell reference atlas of 444 individuals, and 2.2M cells! biorxiv.org/content/10.110… @humancellatlas Here’s a tweetorial on how we built it, and, most importantly, how you can use it: (1/20)

@JarrodShilts @teichlab @bayraktar_lab @StatGenomics @ParsySaeb Not sure if helpful, but LRRC15 in our (healthy) atlas is lowly detected: mostly in peribronchial fibroblasts and lymphatic endothelia. On protein level there might be differences of course, and in health/disease.

@EMadissoon @teichlab @bayraktar_lab @StatGenomics @ParsySaeb Cool to see the additional resolution on lung fibroblast populations! Since you mention COVID19, might be interesting if your data can shine some light on this new mechanism our lab and others found: (biorxiv.org/content/10.110… , biorxiv.org/content/10.110…, & biorxiv.org/content/10.110… )

Our spatial lung and airway atlas with multiple novel cell types and an IgA plasma cell survival niche is out in BioRxiv! Excellent multi-lab team effort @Teichlab @bayraktar_lab, Menna Clatworthy, @StatGenomics, @ParsySaeb, Kerstin Meyer. biorxiv.org/content/10.110…

@jmcdonou @teichlab @bayraktar_lab @StatGenomics @ParsySaeb We think that the newly defined "immune recruiting fibroblast" (localised to immune infiltrate) are on their way to become iBALT or at resolving this structure. So far, we cannot define a link from gland immune niche to the iBALT, but would be super interesting to study.

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb Altogether we use the spatial multi-omics lung atlas to show the gland-associated lymphoid niche (GALN) for IgA plasma cell survival in the airways.

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb Annotation of the submucosal gland (SMG) duct cells in humans allowed to study the cell-cell interaction signals to find recruitment (CCL) and maturation (BAFF/APRIL, IL6) signals between the SMG, T and B-cells.

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb While interesting to see high resolution of immune cell populations, we are especially excited to see IgA plasma cell enrichment in the airway sub-mucosal glands (SMG). Also, IgA plasma cells are increased in covid nasal and tracheal samples, exemplifying the relevance today.

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb We distinguished between systemic and pulmonary circulations for endothelial and especially perivascular cells. Exciting to see similarities between germinal centre postcapillary venous pericytes, and our chemokine-expressing venous perivascular cells (IR-Ven-Peri).

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb We also describe four peripheral nerve cell types in the airway nerves: myelinating and non-myelinating Schwann cells, epineurial and endoneurial fibroblasts. We point out EVX1 motif in myelinating Schwann cells, and show specific expression of rare neuropathy-associated genes.

@teichlab @bayraktar_lab @StatGenomics @ParsySaeb We first created a single cell and single nuclei RNAseq, VDJ-seq and Visium ST dataset from 5 locations of the human lungs. Altogether almost 200,000 cells annotated to 77 cell types/states.

Cambridge Science Festival has been subsumed into @Cambridge_Fest , and we're bringing an hour of science themed comedy to show how a scientist's life can be just as bewildering as anyone else's. Join us Thu 1st April 20.30 - pay what you want at eventbrite.co.uk/e/the-variable…

Celebrating International Women's day from home (11th month in a row). This year's greatest achievements: discovering new cell types in the human lung, organising and performing online science comedy, struggling with chronic pelvic pain and having a baby! #WellcomeGenomeWomen