Edalat Radfar

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Edalat Radfar

Edalat Radfar

@EdalatR

PhD of biomedical optics something. Microscopy, imaging, and so on! :)

UK Katılım Ocak 2015
149 Takip Edilen49 Takipçiler
Edalat Radfar retweetledi
davide calebiro
davide calebiro@DavideCalebiro·
Our @COMPARE_UoBUoN Advanced Microscopy Facility in Birmingham is hiring! Great opportunity to join our vibrant team and lead our state-of-the-art facility! @unibirm_CMH @UoB_MSS
Centre Of Membrane Proteins and REceptors@COMPARE_UoBUoN

We are seeking an Advanced Imaging Specialist at @COMPARE_UoBUoN in Birmingham! Exciting opportunity to lead our #Advanced #Imaging #Facility that houses 9-state-of-the-art commercial #microscopes and several custom systems! #Micorscopy #Job @unibirmingham @unibirm_CMH @UoB_MSS

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Edalat Radfar retweetledi
davide calebiro
davide calebiro@DavideCalebiro·
Great permanent #advanced #microscopy #facility #job @COMPARE_UoBUoN @UoB_MSS @unibirmingham! Don't miss the opportunity! Applications close on 20th January.
Centre Of Membrane Proteins and REceptors@COMPARE_UoBUoN

Exciting #Advanced #Imaging #Specialist position @COMPARE_UoBUoN, @unibirmingham! Join our vibrant biomedical research community to lead our #Advanced #Imaging #Facility, housing 9 state-of-the-art fluorescence #microscopes and dedicated HPC facilities!

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Edalat Radfar
Edalat Radfar@EdalatR·
@RetoPaul If you mean removing the primary objective and inserting a parallel beam, yes, I do. However, with more than 6 degrees of temperature fluctuations per day, it's very unstable.
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Reto Fiolka
Reto Fiolka@RetoPaul·
@EdalatR Have you checked how your detection PSF looks like under flood light illumination? I use that to detect any signs of spherical aberration.
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Edalat Radfar
Edalat Radfar@EdalatR·
Dear Snouty Group, #SnoutClub Despite multiple checks and reasonably precise alignments, I think my setup isn't performing well in terms of axial resolution and light sheet sectioning. In high NA illumination, it's usually about 1.3µm, in the red channel. We expect less than 1µm!
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Edalat Radfar
Edalat Radfar@EdalatR·
@astormic When it's upright, the FWHM is about 1 µm. I'm not sure how to measure it after bending!
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Edalat Radfar retweetledi
Centre Of Membrane Proteins and REceptors
Centre Of Membrane Proteins and REceptors@COMPARE_UoBUoN·
Last chance to join our vibrant COMPARE Microscopy Facility as #Advanced #Imaging #Specialist! Unique opportunity to run a state-of-the-art facility hosting 9 commercial and custom systems (TIRF, SIM, lattice, diSPIM, FRET, etc.)! The post is permanent. Apply by Monday 11th Nov!
Centre Of Membrane Proteins and REceptors@COMPARE_UoBUoN

We are seeking an #Advanced #Imaging #Specialist at @COMPARE_UoBUoN in Birmingham! Exciting opportunity to lead our Advanced Imaging Facility that houses 9 state-of-the-art commercial #microscopes and several custom systems, open to the wide biomedical research community!

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Jonas E
Jonas E@Jonas_Euchner·
Exciting news! On the 23rd of February, I successfully passed my PhD viva with minor corrections, and the examination report attested to a "performance in the top 5%". As of yesterday, my corrections were approved, marking the completion of my doctoral journey. 🎓
Jonas E tweet mediaJonas E tweet mediaJonas E tweet media
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Reto Fiolka
Reto Fiolka@RetoPaul·
@EdalatR @AmirSTORMic @AndrewGYork You can squeeze more photons through, but your Strehl ratio will go down. With Alfred’s zoom lens, we can vary the mag, and if we go off as you propose, the PSF may be ok at the nominal focal plane, but goes quickly downhill at depth. cc @bingy_C , maybe I miss smth.
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Edalat Radfar
Edalat Radfar@EdalatR·
1) Optimizing #OPM🔬 for effective NA=1.5 (or 1.35 silicone) Achievable with some tweaks!🧐 Instead of matching Mrr to primary objective RI (n), consider raising Mrr to ~1.2 x n. Then increase #Snouty's tilt angle a little bit (maintain light-sheet angle at 30° in the sample).👇
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Edalat Radfar
Edalat Radfar@EdalatR·
@RetoPaul @AndrewGYork Indeed, that's accurate. Considering axial magnification about M^2 /n , I think we can compensate the angles mismatches. Corrections may be needed for the actual tilt angle, and there is a possibility of aberrations at depth. But, could remain acceptable within a 10um range.
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Reto Fiolka
Reto Fiolka@RetoPaul·
@EdalatR @AndrewGYork For high NA systems, I would not deviate from the Botcherby-Wilson condition for remote focusing. In your proposed setup, AFAIK, angles from O1 are not matched to the same angles in O2, which would lead to aberrations, especially at depth.
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Brandon Scott
Brandon Scott@MinesBioImaging·
#snoutonauts where are you putting your galvo mirror? I get that it’s in the infinity space, but feels like it needs to be at the focal points to maintain telecentricity. #halp
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Edalat Radfar
Edalat Radfar@EdalatR·
@amsikking Yeah, I like the idea of the AIRR microscopy, but at the moment, we're considering a silicone immersion objective. We should post new nice photos soon after I clean up the table😅 twitter.com/COMPARE_UoBUoN…
Centre Of Membrane Proteins and REceptors@COMPARE_UoBUoN

'Snouty' being tested with their secondary (nicknameless) lens partner for the #MidlandsOpenBioimaging automated 4D microscope, under construction by @EdalatR @IMSR_UoB @unibirmingham .A delicate set up: only 0.3mm between them-hope they remain friends! @BBSRC @InnovationMids

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Keşfet

@COMPARE_UoBUoN @unibirm_CMH @UoB_MSS @unibirmingham @RetoPaul @wellcometrust @Jonas_Euchner @JohnDieSchere