Fraser Johnson

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Fraser Johnson

Fraser Johnson

@FJ_Scientist

🇨🇦 Postdoc at Netherlands Cancer Institute 🇳🇱 Lindeboom Lab 🧬 Stepping into the TCR-pMHC interaction space—one experiment at a time 🧪

Amsterdam, Netherlands Katılım Ağustos 2011
1.1K Takip Edilen454 Takipçiler
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Samuel Hume
Samuel Hume@DrSamuelBHume·
Novartis' new malaria treatment cured 97.4% of patients – more than the current best treatment It kills resistant parasites, too, and probably blocks transmission better than current drugs Approval is expected next year!
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Supratim Datta
Supratim Datta@taurine__·
The seminal paper of Shimon Sakaguchi published in 1995 @J_Immunol where he discovered CD4+ CD25+ cells that actively suppress autoimmunity. It was the first time T regulatory cells appeared in the literature. Not all Nobel Prize winning work need to be in glamorous journals.
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Klebanoff_Lab
Klebanoff_Lab@KlebanoffLab·
🤯Three (!!!) new papers today in @ScienceMagazine on the application of generative AI for the de novo design of peptide/HLA binding molecules! Completely unique 3D structure and binding mode compared with natural TCRs and TCR mimics! Links to papers 👇
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Ly Vu
Ly Vu@Vuphuongly·
Excited to share our first study using nanopore direct RNA seq to comprehensively characterize RNA features. We believe that our deep and high coverage datasets should be a nice resource for the field! cell.com/cell-genomics/…
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KMcNagny-Lab, Biomedical Miracles at work
KMcNagny-Lab, Biomedical Miracles at work@KMcNagnylab·
Canada's first purpose built Biomedical Engineering building. So great to see a timely win for those who believe in the benefits of science and translation and their ability to improving lives.
UBC Engineering@ubcengineering

Welcome home @SBME_UBC students! We officially welcome you to the Gordon B. Shrum Building. With brand new teaching facilities, labs, makerspaces, studios and more, we're eager to see how you use this space to innovate. Learn more: bit.ly/4jk2Fk3

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Feng Zhang
Feng Zhang@zhangf·
We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their DNA targets. science.org/doi/10.1126/sc…
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Tanentzapf Lab
Tanentzapf Lab@TanentzapfLab·
Reading a nice polished CV is useful & everything but the most important part of my scientific training, the 18 months I spent on a project that failed, which taught me resilience, problem solving, humility, & other such wisdom is nowhere to be found on my CV. So CVs have limits
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Adam Grant
Adam Grant@AdamMGrant·
How to be a better critical thinker: 1. Be 10% more skeptical of people you agree with—and 10% more charitable to people you disagree with 2. Look for flaws in ideas you like—and strengths in arguments you dislike 3. Learn from sources that engage with competing ideas
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Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
@tungnguyen Nah im an amateur biologist with a home lab and nothing more. Just this lil redearch nook in the hallway of my home :)
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Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
Boiler clogged and was spewing carbon monoxide gas. Coulda died. Just got hit with a $10,000 bill so savings wiped. If y'all wanna buy some chromoprotein plasmids, all proceeds go to paying this off. Gonna add a Golden Braid entry vector kit soo. Atinygreencell.com
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Sjoerd Slagman
Sjoerd Slagman@SjoerdSlagman·
@ATinyGreenCell The @WUR has the University Fund Wageningen which also has funds specifcally aimed at people from the global south. On top @WUR is absolutely top-notch when it comes to biotech. Yes... I am biased The fund can be contacted through this link: wur.nl/en/value-creat…
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Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
I have a pen pal from Uganda looking to study abroad. He's 21 years old and interested in biotechnology. Do y'all have any resources for African students looking for scholarships? TYIA!
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Fraser Johnson
Fraser Johnson@FJ_Scientist·
@ATinyGreenCell I prepared and vacuum filtered 500 mL of TSS buffer, aliquotted and froze it down. I'll add 3 uL bacteria to 3 uL cold DNA, raise the volume with 30 uL cold TSS buffer and proceed as normal. Substituting TSS for LB in that step failed to produce colonies so I'm sticking to TSS.
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Fraser Johnson
Fraser Johnson@FJ_Scientist·
@ATinyGreenCell My variation was to grow up 400 mL intraday (aiming to hit 0.35-0.4 OD600 but not critical if overshot), resuspend in 40 mL TSS, aliquot into 80 tubes of 500 uL. I'm finding the that 3-5uL bacteria consistently give me colonies and they're pretty resistant to freeze thaws so far.
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Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
It's such a simple protocol that I invite anyone to please try and report back conditions as well as CFU/mL. Could be fun to crowdsource optimizations. A lil community project that benefits anyone working in microbes :)
Sebastian S. Cocioba🪄🌷@ATinyGreenCell

Want decent competent cells with at least 10^6 CFU/mL but don't feel like stressing? Try this: 1. Grow ecoli overnight 37C in LB shaking 250rpm 2. Spin down 1mL 15sec 10,000xg 3. Decant and resuspend in 500uL of ice cold TSS buffer. Vortex to resuspend in 3 pulses 1 sec. Done

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Keşfet

@J_Immunol @ScienceMagazine @GenomeBiology @oncodeinstitute @NKI_nl @NatureMedicine @bencoates1 @ATinyGreenCell