Fanara Steven

📢 Preprint alert! We investigated how citrate loading into xylem vessels and nicotianamine (NA) compartmentation jointly regulate metal homeostasis in Arabidopsis thaliana. doi.org/10.64898/2026.… A little thread...

#TansleyInsight: CLE peptides in plant-biotic interactions Nicolas Frei dit Frey & Thomas Spallek 👇 📖 nph.onlinelibrary.wiley.com/doi/10.1111/np… #LatestIssue

HUGE thanks to the Translational Plant Biology team, Prof. Marc Hanikenne for his guidance, and all colleagues who contributed to this work through experiments, laughs and/or discussions. ❤️

9/ Overall, our results show that simultaneous disruption of citrate export and NA compartmentation leads to major alterations of the ionome, impaired metal partitioning, persistent oxidative stress and defects in RAM function.

8/ While all the other genotypes produce siliques under hydroponic conditions, the double mutant fails to complete reproductive development. Seeds were only obtained from soil-grown plants supplemented weekly with sequestrene, and they show elevated Fe and Zn levels.

📢 Preprint alert! We investigated how citrate loading into xylem vessels and nicotianamine (NA) compartmentation jointly regulate metal homeostasis in Arabidopsis thaliana. doi.org/10.64898/2026.… A little thread...

7/ These phenotypes are associated with strongly reduced root-to-shoot translocation, despite the pronounced induction of the NA biosynthetic pathway, which likely reflects an attempt to restore metal mobility.

6/ At later vegetative stages, these trends persist: high Fe, Mn and Zn accumulation in roots and shoots, and sustained oxidative stress in roots.

5/ We observed strong accumulation of Fe, Mn and Zn in the double mutant, together with the enhanced activity of the Fe uptake machinery, including IRT1 that is known to uptake these metal ions.

4/ Interestingly, LR density increases under Zn excess, primarily due to the severe inhibition of the PR elongation. This prompted us to examine the integrity of the root apical meristem (RAM), which is reduced in the double mutant and shows visible cell death under high Zn.

3/ The double mutant exhibits strong alterations in root system architecture, exacerbated under high Zn stress: shorter primary root (PR), and fewer and shorter lateral roots (LR).

2/ To address this, we generated frd3 zif1 double mutants and assessed several traits, including developmental parameters and ionomics, across young and mature vegetative, as well as reproductive stages (kinda, stay until the end! 😉)

1/ Citrate and NA have distinct but interconnected functions. a) Citrate supports metal transport in xylem-fed tissues b) NA is involved in intracellular trafficking and phloem-mediated remobilization. Their coordination remains poorly investigated at the physiological level.

1/ ✨ New preprint alert ✨ "Stepwise evolution of the developmental and symbiotic functions of DELLA in land plants" Here we investigated the role of the single GRAS transcription factor DELLA in #MyMarchantia doi.org/10.64898/2026.… #PlantScience A thread...

@583original Thank you Satoshi!

Congratulations, Steven!

@Amandine_Brtrd @MSCActions Thank you! ❤️

@FanaraSteven1 @MSCActions Congratulations Steven! I’m happy for you! I’m curious to read the next chapter of your research

My proposal was one of 1,966 submitted in the MSCA-LIF (Life Sciences) section this year. Despite my initial doubts, I am beyond thrilled to have been awarded this grant. I am truly honored to have incredible supervisors who guide me in the right direction. @MSCActions

I feel incredibly lucky to be surrounded by inspiring and driven colleagues and friends. Science has never felt so fulfilling and balanced for me!

It's my pleasure to share with you a chapter of the project I fell in love with five years ago 🍀🦠 biorxiv.org/content/10.110… Here is a thread 🧵

Nucleo-cytoplasmic distribution of SAP18 reveals its dual function in splicing regulation and heat stress response in Arabidopsis #research #PlantCommunications cell.com/plant-communic…

Our new preprint! 🌱 We identified four new AtZIP transporters localized at the root outer domain which contribute to metals acquisition from the soil! biorxiv.org/content/10.110…