Hichrom Limited

144 posts

Hichrom Limited

Hichrom Limited

@Hichrom

HPLC column manufacturer and supplier of the world's largest range of hplc columns. HPLC Technical support is our speciality!

Theale,UK Katılım Mayıs 2009
18 Takip Edilen187 Takipçiler
Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC Gold rule of LC .... other parameters eg sound of the system, are rarely recorded yet can give important clues to troubleshooting..
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC Gold rule of LC .... Some of the operating parameters eg flow rate & pressure are easy to quantify.....
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC... why HILIC.... IC - high salt concentration? Non charged species? Compatibility with MS & ELSD?
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC-......"pure silica" ... extremely low metal content; best materials for the analysis of bases.
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC-......"pure silica" ... extremely low metal content; best materials for the analysis of bases.
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC-"pure silica" produced via the polymerisation of tetraethylorthosilicate; uniform associated silanol groups are produced .......
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC Remember.. if you are out by 1% in your mobile phase composition.. that can equal a HUGE 5% change in retention time
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC - ...... ENCAPPING is used to reduce the no. of isolated silanols and only 30% of available silanols are capped!!!
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC-ENDCAPPING is performed after bonding C18 onto silica;......
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC-"base deactivated" silica is acid treated to reduce metals which are responsible for increasing the acidity of the silica surface
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC -How to overcome the problem of peak tailing: solution 3 - use PURE silica!
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC -How to overcome the problem of peak tailing: solution 2 - use "base deactivated" silica!
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Hichrom Limited
Hichrom Limited@Hichrom·
#HPLC -How to overcome the problem of peak tailing: solution 1 - ENDCAPPING!
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Hichrom Limited
Hichrom Limited@Hichrom·
Not-so-new to #HPLC? then Dr John Dolan is running 2 courses 13/14th May, in Manchester: HPLC MD & Validating Methods - there is time!
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Hichrom Limited
Hichrom Limited@Hichrom·
All the details of the Prof Mel Euerby & Dr John Dolan Hichrom #HPLC courses can be found here. htm ow.ly/ITjP3
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