Karthik Subramaniam Pushpavanam

A fun example of proteins engineered beyond biology → sustainable nanoparticle recovery. Read more: pubs.acs.org/doi/full/10.10… @ACS_Langmuir

Silica nanoparticles are everywhere, but recovering them from water remains a challenge. In our latest work, engineered supercharged GFP proteins act like molecular “bridges” to capture and aggregate silica nanoparticles entirely in water.

Big shout-out to Vidhi and Gaurav Chauhan from IIT Indore, whose coarse-grained simulations revealed the underlying mechanisms and strengthened our understanding.

What makes this exciting is that it avoids emulsion-based or microfluidic methods, which often use oils or have limited throughput. Instead, it relies on intrinsic protein interactions — a gentle, biocompatible, and scalable route to microgel fabrication. 🔗

Our recent work, “Liquid–Liquid Phase Separation to Fabricate Microgels of Recombinantly Expressed Proteins,” is finally out! Read the paper: lnkd.in/dA58Q9Jv

Our paper in Communications Biology (rdcu.be/eDDFZ) shows that a tiny 9-aa fragment from a light-cleaved protein doesn’t go dark — it aggregates and glows dim red. Led by Yashwant Kumar & Krishna Agrawal. Grateful to @ANRFIndia for support!

Our latest from the lab — led entirely by students Aniruddha Kambekar, Vignesh Nandhagopal, Aditya Mehta & Yashwant Kumar (PhD)! They built dual-layered hydrogels with spatially organized Au & Ag nanoparticles Catalysis + photothermal in one. 🔗 pubs.acs.org/doi/full/10.10…

Our manuscript in @Biomaterials_ Histamine Receptor Agonism Differentially Induces Immune and Reparative Healing Responses in Biomaterial-facilitated Tissue Repair #skinsealing sciencedirect.com/science/articl… @NIAMSFunding @NIBIBgov @ASUBiodesign @ASUResearch @SEMTEatASU @jordanyaron

We welcome all interested students, whether you have cleared DBT-JRF, CSIR-NET, or INSPIRE fellowships, or have a passion for protein design and related fields to discuss PhD opportunities in our lab (kpsubram[at]iitgn.ac.in).

Our research combines advanced molecular techniques to develop proteins with real-world applications in biosensing, environmental monitoring and therapeutic development.

Are you passionate about protein engineering? We are looking for PhD candidates with an interest in molecular biology, protein engineering, and biotechnology.

This Teacher’s Day, my students surprised me with something truly special – they wrote about the regular phrases I use in our daily interactions. It’s incredible to see how something I say casually has become a (hopefully) lighthearted part of their day.

Our studies show LAMP can achieve over 90% purity, offering a promising alternative to conventional methods. This work was spearheaded by Yashwant Kumar, Krishna Agrawal and Manisha Ojha. We would like to thank @gsbtm for their funding support.

Traditional methods, like resin-based chromatography, face challenges such as nonspecific binding and complex elution buffers, which further enhance the final cost. This stimuli-responsive technique leverages light to purify proteins with precision.

The production of recombinant proteins is central to biotech, yet downstream processing remains a major bottleneck. To tackle these issues, we’ve developed a light-activated molecular purification (LAMP) system using a photocleavable protein. Explore LAMP pubs.acs.org/doi/10.1021/ac…

Submission Link: app.jove.com/methods-collec… There are also fee waivers and discounts for select nations including India.

Excited to guest edit a Methods Collection for @JoVEJournal on high-throughput materials discovery in biomaterials! We invite submissions on experimental techniques, data analysis, automation, and more. Let's accelerate biomaterial discovery for biomedical breakthroughs!

This work addresses this critical issue by offering a sustainable and efficient solution for REE recovery.

REEs are indispensable in various applications, including smartphones, electric vehicles, and clean energy technologies. However, their selective purification poses significant challenges due to their similarities in physico-chemical properties.

Delighted to share our latest research in sustainable resource management! pubs.acs.org/doi/full/10.10… Hrishitha Sree and in collaboration with @Sgbiolab, have utilized immobilized lanthanide binding peptides for selective rare earth element recovery.