Korostelev Lab

All life depends on accurate translation (both intracellular & international). Using time-resolved cryo-EM, we watched ribosomes decode mRNA, aided by EF-Tu and GTP. See our animations & article (nature.com/articles/s4158…) on how ribosomes proofread tRNAs to make correct proteins:

The Gonzalez Lab at Columbia University is seeking a scientific leader! Join us in a Research Scientist position, driving single-molecule, structural, and biochemical studies of translation, translational control, and other RNA-based processes. Apply: apply.interfolio.com/176949

@zegarrv Thank you.

@KorostelevLab Congrats!!

GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome. GTPBP1 promotes more stringent mRNA decoding than the bona fide delivery factor eEF1A. Congrats to Denis Susorov and collab with Pestova lab! tinyurl.com/3smdvt3v

Using "in extracto cryo-EM", we visualize ribosomes in mammalian lysates, including RRL. "Hibernating" ribosomes carry proteins that protect functional centers. These include elongation factor eEF2, LARP1 implicated in mTOR signaling, eIF5A etc. biorxiv.org/content/10.110…

Preprint from our collaboration with the labs of Gerry Wright and Shura Mankin @shuramankin identifying the first translation inhibitor binding to the E-site of the bacterial ribosome… researchsquare.com/article/rs-692…

@slavicaPD @Hashemyaser4 Thank you Slavica!

@KorostelevLab @Hashemyaser4 Fantastic!

ROOL monomers entangle with other monomers via strand swapping to form octameric nanocages. nature.com/articles/s4158…

@disney_lab Thanks Matt. RNA is amazing indeed.

@KorostelevLab amazing!

With our RTI colleagues Xiaobin Ling and @WenwenFang2, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of long-noncoding ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al! nature.com/articles/s4158…

Activation of Retron-Septu for antiphage defense. Congrats @futianmin and all co-authors! science.org/doi/full/10.11…

“Undermining the future of American science is not a path to accountability — it’s a path to decline.” In a powerful new piece, my colleague Daphna Shohamy explains how canceled grants are driving young scientists out of science—and out of the country. 🔗 statnews.com/2025/05/07/us-…

In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…academic.oup.com/nar/article/53…

We developed a new siRNA drug candidate for prion disease, a @broadinstitute / @RTI_UMassChan collaboration. We now have an open IND meaning FDA has given us permission to start first-in-human trials. Work remains before trials can launch. The full update: cureffi.org/2025/04/14/ope…

I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex. jbc.org/article/S0021-…

Fresh off the press…our latest study on the Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit | great collaboration with @inada_lab and Zaher lab. Data collected at @DCI_EM. PNAS pnas.org/doi/10.1073/pn…

Anastasia Khvorova, PhD, named 2025 Else Kröner Fresenius Prize for Medical Research awardee for her pioneering work in the field of RNA-based therapies: direc.to/nacN #EKFprize25 #EKFS #ElseKrönerFreseniusStiftung #RNA #RNAtherapies @EKFStiftung @RTI_UMassChan

Biological systems find ingenuous solutions. Almost anything that can happen, happens. Still, I would not have guessed a role of RNA-binding proteins on the cell surface. The Flynn lab are boldly investigating glycoRNAs and following their leads.

Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation cell.com/cell/fulltext/…

Our review on small endogenous RNAs in animals is online. We thank @MartinjSimard, @SvobodaLab and the third, anonymous, reviewer for helping us improve our manuscript. bit.ly/3Ebk931

Gabriel Demo and colleagues report how a small archaeal protein (aRDF) dimerizes 30S subunits to prevent ribosome formation: academic.oup.com/nar/article/53…