Krezel Lab

New Article in Communications Chemistry (Nature). nature.com/articles/s4200…

Over the next 4 years, thanks to @NCN_PL funding, we will explore "Principles of hidden zinc bioinorganic chemistry: Tuning sequence-structure-stability relationships for improved zinc protein functionality".  The PI of the project is @artur_krezel. #Opus #zinc

Biarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets sciencedirect.com/science/articl…

An efficient and easily obtainable butelase variant for chemoenzymatic ligation and modification of peptides and proteins …robialcellfactories.biomedcentral.com/articles/10.11…

Site-Specific and Fluorescently Enhanced Installation of Post-Translational Protein Modifications via Bifunctional Biarsenical Linker | ACS Omega pubs.acs.org/doi/10.1021/ac…

Antioxidant-related enzymes and peptides as biomarkers of metallic nan... sciencedirect.com/science/articl…

Combining Native Mass Spectrometry and Proteomics to Differentiate and Map the Metalloform Landscape in Metallothioneins | Journal of Proteome Research pubs.acs.org/doi/10.1021/ac…

Phytochelatins Bind Zn(II) with Micro- to Picomolar Affinities without the Formation of Binuclear Complexes, Exhibiting Zinc Buffering and Muffling Rather than Storing Functions | Inorganic Chemistry pubs.acs.org/doi/10.1021/ac…

Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3 …robialcellfactories.biomedcentral.com/articles/10.11…

Structural Characterization of Cu(I)/Zn(II)-metallothionein-3 by Ion Mobility Mass Spectrometry and Top-Down Mass Spectrometry | Analytical Chemistry pubs.acs.org/doi/10.1021/ac…

Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn academic.oup.com/metallomics/ar…

A combined biochemical and cellular approach reveals Zn2+-dependent hetero- and homodimeric CD4 and Lck assemblies in T cells: Structure cell.com/structure/pdf/…

A new article on mammalian zinc metallothioneins in Metallomics has just been published. "The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties"academic.oup.com/metallomics/ad…

From methodological limitations to the function of metallothioneins – A guide to approaches for determining weak, moderate, and tight affinity zinc sites academic.oup.com/metallomics/ad…

Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of zinc metallothionein-2 species - now published in Chemical Communications pubs.rsc.org/en/content/art…

OaAEP1 Ligase-Assisted Chemoenzymatic Synthesis of Full Cysteine-Rich Metal-Binding Cyanobacterial Metallothionein SmtA pubs.acs.org/doi/10.1021/ac…

Our newest work on Zn(II)-dependent CD4 and Lck assemblies in T cells: A combined biochemical and cellular approach reveals Zn2+-dependent hetero- and homodimeric CD4 and Lck assemblies in T cells biorxiv.org/content/10.110…

We are happy to share our new study about synthesis of homogeneous site-specific Ub-peptide conjugates by a sortase mediated chemoenzymatic strategy, presented in @ACS_Omega by Singh et al. @Avi598 @artur_krezel pubs.acs.org/doi/10.1021/ac…