Link Lab

@ACSPublications Thanks @ACSPublications for selecting our article as Editors Choice.

'The Metabolic State of E. coli Influences Fosfomycin Efficacy and Promotes Resistance Evolution' from ACS Infectious Diseases is an #OpenAccess #ACSEditorsChoice. 📖 Read the article: go.acs.org/dQV

Technically this required metabolomics on ~3,000 samples from an arrayed CRISPRi library. The bottleneck wasn’t MS or sampling. It was sequencing the strains after arraying. Paper: cell.com/cell-systems/f…

Example: Knockdown of ispB increases farnesyl-PP. Add a carotenoid pathway and get more product. 🟠 Metabolite accumulation can reveal engineering targets.

What happens if you knock down every metabolic gene in E. coli? We measured the metabolome of 1515 CRISPRi strains. Result: metabolism behaves like traffic. Block an enzyme and metabolites pile up upstream. 🚗🚗🚗 New paper 🧵

New paper from our group: Metabolism Influences Fosfomycin Efficacy pubs.acs.org/doi/10.1021/ac…

PhD and Postdoc positions opening in synthetic biology, CRISPR genome engineering of bacteria, and metabolomics. Details and contact here: linkmetabolism.com/join-us

Preprint from our lab: In synthetic nasal communities a single Corynebacterium propinquum strain can exclude Staphylococcus aureus through nutrient competition. biorxiv.org/content/10.648…

We are searching PhD students in synthetic biology and metabolic engineering uni-tuebingen.de/universitaet/k…

Great collaboration with the @sourjik lab. Check out how isoprenoid biosynthesis, high oxygen and a membrane complex influence E. coli fitness 👇

Our CRISPR screen in E. coli shows that metabolic mutations influence antibiotic action. Pathway-specific bottlenecks cause resistance. Slow growth leads to tolerance. Read more 👉 doi.org/10.1038/s44320…

New paper from our group: we developed intact protein barcoding, which uses small proteins as a barcode to identify CRISPRi strains with mass spectrometry. search.app/Tb7XiPbBoj1rnJ…

Congratulations Dr. Lubrano 🥳

Our group was featured in the key to my research podcast. There is a short version and a deep dive about our research and episodes with other great scientists from @uni_tue, @Gh_Tabatabai, @EKemen. Check it out: keytomyresearch.podbean.com

Don't miss the #GCSB Conference⏰!  With less than a month to go, it's time to secure your spot and explore the latest advancements in synthetic biology📅. Check out the now published poster program and get your tickets right here👇 gcsb2024.de

Two-stage bioprocesses could overcome many limitations in bioengineering. Check out our review about this exciting topic👇 Led by @paulsimv. Thanks for the nice collaboration. nature.com/articles/s4422…

@JorgStulke @WarnekeRobert @ThorbenSchramm @JBacteriology @uniGoettingen @uni_tue @SPP1879 Great work and thank you for the collaboration

News on the PII-like protein DarA! Great work by @WarnekeRobert in collaboration with @LinkLabs @ThorbenSchramm @JBacteriology @uniGoettingen 🤝 @uni_tue Thanks to @SPP1879 journals.asm.org/doi/10.1128/jb…

Thanks @VanessaPahl , @paul_lubrano92 and Daniel Petras @func_metabo_lab

Check out our new method to read library barcodes and measure metabolites of CRISPRi strains with #massspec. Works almost in real-time: biorxiv.org/cgi/content/sh…