Plant Physiologist

@bioprotocolbyte paper. We present instructions for adapting two LI-6800 to simultaneously measure adaxial and abaxial gas exchange. We included details of the apparatus, scripts, and how to estimate the boundary layer conductance for this setup @LICOR_ENV doi.org/10.21769/BioPr…

Release: StomaAI (SAI) StomaAI (SAI) is a third-party app for auto-measuring stomatal density and pore features (width, length and area) from microscope images with human-level accuracy via deep computer vision. plantphysiologist.com/utilities

@pepcanadell @prabir_patra It is really shocking. Thanks for sharing. Here a comparison in the last 850,000 years. The red dot is an arbitrary day in December 1980 for reference between graphs.

Thanks @prabir_patra for putting together this figure for the chapter.

Thread 1/6 How do we know for sure that the growth in atmospheric CO2 is due to human activities, & not due to volcanoes, geological seeps, wildfires, decomposition of permafrost C. It might sound like a rhetorical question in 2022 but the evidence is great C cycle sciences.

Human activity has triggered an overwhelmingly rapid increase in the atmospheric [CO₂], noticeable in our lifetime, e.g. Darwin was born at 284 ppm, R. Franklin at 302 ppm and today we are at 419 ppm. What was the [CO₂] when you came into the World? plantphysiologist.com/CO2_at

We are advertising a 2 year postdoctoral fellowship at the ANU node of C4 rice aus01.safelinks.protection.outlook.com/?url=https%3A%…

@Lehmann_Lab @torresruizjm Very interested, I send you a message

@P_Physiologist @torresruizjm I have stomta density data from more then 80 species of various functional groups. Interested?

Release: Stomatal density repository We are starting with a collection of 384 species. The repository is always growing and we welcome anyone sharing their published information. To include your data, contact us. plantphysiologist.com/RepSr

@FXPicoMercader The idea is to generate a repository with multiple entries for the same species (references), including variations on growth conditions, environmental conditions, lab, field, etc. In practice, any publication containing stomatal density data.

@ClaudioMonteza @odealab Were you discussing microscopy or stomatal density? This picture is a section of the abaxial surfaces of a Capsicum annuum leaf.

This stomatal pic reminds me of a Tropical ecology weekend course taught by @odealab et al, in Galeta marine station🙂. Only 13 years ago 😆 Intense course, we conceptualized research ideas, collected data & presented before end of weekend. Like having an espresso macchiato 😂.

@keholsinger @nora_flora10 @KristenNolting @T_Marie_Wms We are happy to add more data to the repository. If anyone is interested in adding their information they can contact us on the web or via Twitter

I know some people with data that could be shared @nora_flora10 @KristenNolting . Did @T_Marie_Wms measure stomatal density? Maybe she could convince her Pelargonium friends to share.

Update: Add-on MSF calculations for LI6800 version 1.1 Correction in the equation to calculate ws plantphysiologist.com/18

"…they turn the standard method for calculating stomatal conductance and intercellular CO2 concentration from gas exchange measurements on its head." Red light shines a path forward on leaf minimum conductance 👇 nph.onlinelibrary.wiley.com/doi/10.1111/np… Commentary by @LucasCernusak @mdekauwe82

Release of a self-developed add-on for LI6800. The add-on includes calculations of gas exchange parameters accounting for small fluxes as presented by Márquez, Stuart-Williams, and Farquhar (2021) (MSF). Find it in: plantphysiologist.com/utilities

Recently accepted in New Phyt 📖 🌱 @DiegoMa_rquez et al. #Leaf surface conductance ow.ly/8xbd50FstUE 🌱Feng et al. Plant potassium uptake ow.ly/VSc950FstUF 🌱 @WernerMayerhofe et al. Ectomycorrhizal association with Fagus sylvatica ow.ly/g9KN50FstUD

Paper accepted in @NewPhyt. Our paper discusses the adaxial/abaxial cuticular conductance (gcw), presents a technique to estimate gcw and an app to help with the calculations (see @P_Physiologist) @GrahamDFarquhar @FlorianABusch @PlantSci_ANU @leaf2field nph.onlinelibrary.wiley.com/doi/abs/10.111…

Release of gcwCi web-app The web-app gcwCi helps to estimate the cuticular conductance to water of a leaf surface using normal gas exchange parameters, requiring as an input a known CO2 concentration within the leaf (ci). plantphysiologist.com/gcwCi