Rory Power

@RetoPaul Something seems off though: "Without the water, reaching an NA of more than 1 was impossible. With it, our systems' optics reach an NA of 1.35". Presumably there is something in the water to reach n > 1.35?

5nm feature size with 13.5nm extreme UV requires a numerical aperture (NA) above 1. They use water immersion lenses (mirrors) to get there... asml.com/en/technology/… 😎

@RetoPaul If you ignore a minus sign, the day has already provided one treat at 1:38 10/(-)23 one gets Boltzmann's constant... I'm sure many others can be shoehorned in as well. what a time to be alive

Happy Mole day, to anyone who celebrates (6:02 AM 10/23 -> 6.02 *10^23). Have some Guaca-Mole or some just some Avocad(r)o to celebrate.

@adam_k_glaser Assumed that would be the case. Yes indeed, that would be a monster of a tube lens. Very nice nonetheless!

@Rory_M_Power Unfortunately finite. Schneider's lenses are all finite. I think infinity with a tube lens would be possible, but would require a massive tube lens to fill the 82 mm sensors these lenses are designed for!

New TITANITE 5.0X / NA 0.27 microscopy lens now available from Schneider-Kreuznach! Purpose-built ExA-SPIM lens with steel dipping caps in place of the original beamsplitter. Same 16.8 mm FOV, slightly lower WD (22 vs 35 mm) and NA (0.27 vs 0.30), but with dipping capabilities.

Anyone interested in developing light sheet technologies towards real service applications as part of ARISE2, please get in touch. embl.org/about/info/ima…

@kD3AN @zacsimile @RetoPaul Will do Kevin!

Will definitely be testing this at some point. Congrats to @zacsimile @kD3AN @RetoPaul and others.

Well this is nuts science.org/doi/10.1126/sc…

@ZacSwider @anacondainc Anaconda have started requiring that academic and non-profits with more than 200 employees have to purchase a license per user.

@Rory_M_Power @anacondainc How so?

Well I guess I'm just going to need to get better at keeping all my python packages playing nicely together... no worries I'd gotten too lazy... @anacondainc terms and conditions aren't playing so nicely with academic insitutions it seems.

@RetoPaul @Thorlabs @amsikking @Bodeeen1 @bingy_C Thanks! Right, I suppose curvature in image space will not so uniformly aberrate the PSF. Good to know though

@Rory_M_Power @Thorlabs @amsikking @Bodeeen1 We have a couple Vantage Pro 20 and 30mm galvos, in an image space scanning format. We did not notice anything negative about flatness. With the caveat that any curvature in image space would not as directly show up on the PSF as if they were in pupil space. @bingy_C Thoughts?

Anyone been using the large (e.g. 20 mm) @Thorlabs galvos and can comment on flatness? thorlabs.com/newgrouppage9.… Any better options for a large aperture 1D scanner with high flatness? Can sacrifice some speed. no problem

@Thorlabs Thanks! Sure, I will reach out. Maybe lambda/10 across @ ca 550 nm. Lambda/8 would probably be fine as well.

@Rory_M_Power How flat are you looking for @Rory_M_Power?  If the optics that come on our standard options don’t meet your requirements, we would be happy to discuss a custom mirror for you! Reach out to us at techsales@thorlabs.com.

@adam_k_glaser Would be wonderful if they (or others) could develop something that is infinity corrected with a paired tube lens and without requiring additional refractive media between the lens and sample.

Exciting to see Schneider-Kreuznach supporting our ExA-SPIM project, and the more widespread use of their industrial lenses for light-sheet / fluorescence microscopy. They engineer incredible lenses that we should pay attention to! Please take a look! schneiderkreuznach.com/en/industrial-…

@amsikking Considered adding this to our OPM but ran out of space in front of the camera with a dual-channel splitter/recombing on a knife-edge mirror... Look at that Argolight Slide rotate though....

#AIRR microscopy, the final touch: Real-time multi-angle projections! -> Get ultra-fast 2D projections of 3D objects of any refractive index, with a light-sheet microscope that uses a single AIR objective at the sample! Crazy!

#FeaturedProtocol this week is a comprehensive guide to building and operating a custom 3D #singlemoleculelocalization #microscope bit.ly/3UyPQYq @Rory_M_Power @JonasRies @EMBLImaging

Everything you need to build your own 3D multi-color, automatable single molecule localization microscope with full biological validation. Plus lots of rainbow CAD! @JonasRies @aline_tschanz @EMBLHeidelberg rdcu.be/dGKt5 nature.com/articles/s4159…

@AmirSTORMic @AleksPonjavic Nice! Need to dig through the details. Have been considering something like this on our OPM with the 100x silicone oil O1