CRISP_SCIENCE

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CRISP_SCIENCE

CRISP_SCIENCE

@ScienceCrisp

RIKEN (CODATA, WDCM ...) - NIG (CODATA, DDBJ/CIB, WDCM, OECD BRC, GBIF, JST BIRD, TPRP ...) - FREE

Tokyo (Web site in Japanese) Katılım Ocak 2017
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CRISP_SCIENCE
CRISP_SCIENCE@ScienceCrisp·
マウス受精卵への大規模な断片 [*1] を精密に挿入する技術を, #CRISPR-Cas9 HDRをベースに確立したことで, ヒト化免疫グロブリン重鎖マウスを極めて短期間で[*2], 利用・評価できるようになりました crisp-bio.blog.jp/archives/40601… [*1] 155-kb, [*2] 8週間
CRISP_SCIENCE@ScienceCrisp

“#CRISPR-mediated precise large fragment insertion in zygotes enables rapid generation of humanized immunoglobulin heavy-chain mice" Nair U, Akauliya M, Warner JE [..] Batista FD [+10]. Immunity 2026-07-09. doi.org/10.1016/j.immu… …Stable insertion of 155 kb of human VH DNA via CRISPR-Cas9 homology-directed repair (HDR) … the humanized mice from CRISPR-Cas9 GE zygote are ready in 8 weeks

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CRISP_SCIENCE@ScienceCrisp·
細菌由来ナノシリンジへのRNA積載を可能にし, ゲノム編集やがん治療への応用可能性を示しました： DART（PVC Docker-based All-purpose RNA Injection DeliveryTool） crisp-bio.blog.jp/archives/40599…
CRISP_SCIENCE tweet media
CRISP_SCIENCE@ScienceCrisp

"Reprogrammable bacterial nanosyringes to deliver RNA and gene editors" Haishan Xu, Lei Feng, Ningning Song, Xiangxiang Zhao, Xianchao Feng. (bioRxiv 2025-10-31) Trends Biotechnol. 2026-06-22. doi.org/10.1016/j.tibt… …PVC (Photorhabdus virulence cassette) …DART（PVC Docker-based All-purpose RNA Injection Delivery Tool）delivers pepper RNA, guide RNA. siRNA, miRNA, mRNA, … DART-mediated "#CRISPR-Cas9 KRAS knockout" produced a significant antitumor effect in a subcutaneous mouse tumor model.

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CRISP_SCIENCE@ScienceCrisp·
“#CRISPR-mediated precise large fragment insertion in zygotes enables rapid generation of humanized immunoglobulin heavy-chain mice" Nair U, Akauliya M, Warner JE [..] Batista FD [+10]. Immunity 2026-07-09. doi.org/10.1016/j.immu… …Stable insertion of 155 kb of human VH DNA via CRISPR-Cas9 homology-directed repair (HDR) … the humanized mice from CRISPR-Cas9 GE zygote are ready in 8 weeks
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CRISP_SCIENCE@ScienceCrisp·
"Reprogrammable bacterial nanosyringes to deliver RNA and gene editors" Haishan Xu, Lei Feng, Ningning Song, Xiangxiang Zhao, Xianchao Feng. (bioRxiv 2025-10-31) Trends Biotechnol. 2026-06-22. doi.org/10.1016/j.tibt… …PVC (Photorhabdus virulence cassette) …DART（PVC Docker-based All-purpose RNA Injection Delivery Tool）delivers pepper RNA, guide RNA. siRNA, miRNA, mRNA, … DART-mediated "#CRISPR-Cas9 KRAS knockout" produced a significant antitumor effect in a subcutaneous mouse tumor model.
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CRISP_SCIENCE@ScienceCrisp·
Review “#CRISPR in clinical oncology: translational advances from molecular diagnostics to therapeutics" Grigg S, Shembrey C, Fareh M, Blombery P, Corn JE, Seymour JF, Casan JML. Nat Rev Clin Oncol 2026-07-09. doi.org/10.1038/s41571… … CRISPR-enabled functional genomics, diagnostics, therapeutic strategies (from ex vivo immune cell engineering to nascent in vivo interventions), and technological and regulatory challenges in oncology.
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CRISP_SCIENCE@ScienceCrisp·
“Optimized prime editing for precise gene modification conferring herbicide resistance in sorghum” Zhou J [..] Li S, Dun B [+9]. Plant Physiol. 2026-05-06. doi.org/10.1093/plphys… #CRISPR
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CRISP_SCIENCE@ScienceCrisp·
"Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing" Price D, Zemlyanskiy G, Trakarnphornsombat W [..] Radzisheuskaya A [+5] Nat Genet 2026-07-08. doi.org/10.1038/s41588… … This study establishes a functional map of histone H3 lysines in mammals and provides a broadly applicable platform for systematic dissection of chromatin regulation. #CRISPR
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CRISP_SCIENCE@ScienceCrisp·
tBE (transformer BE): "Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations" Wang L, Xue, W, Zhang H [..] Yang B, Yin H, Yang L, Chen J. Nat Cell Biol. 2021-05-10. doi.org/10.1038/s41556… … we used a cleavable deoxycytidine deaminase inhibitor (dCDI) domain to construct a transformer BE (tBE) system that induces efficient editing with only background levels of genome-wide and transcriptome-wide off-target mutations. #CRISPR
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CRISP_SCIENCE@ScienceCrisp·
"Efficient in vivo cytosine base editing using virus-like particles with uracil DNA glycosylase inhibition" Zhu J, Ding L, Liu KM, Zhu X, Li J [..] Hong J, Yang L, Chen J [+12]. Nat Biotechnol 2026-07-10. doi.org/10.1038/s41587… ... tBE-VLP4 induced no detectable off-target edits in vitro or in vivo and demonstrated superior specificity compared to AAV or lipid nanoparticle mRNA delivery. Our work establishes tBE-VLP4 as a precise, efficient system for in vivo cytosine base editing. #CRISPR
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CRISP_SCIENCE@ScienceCrisp·
CRISPRは遺伝子だけでなく「タンパク質工場」も制御できる ― Science誌が先行公開したTAPIRによるタンパク質増産 crisp-bio.blog.jp/archives/40581…
バイオステーション(Bio-station)/ポッドキャスト@Bio_stations

リボソームRNAの転写活性化による細胞運命制御 dCas9を用いてPolIをリクルートすることでリボソームRNAの転写を活性化する系を確立。神経幹細胞など幹細胞運命にrRNAの量自体が重要であることを発見。リボソーム病の病態改善やがん細胞の増殖機構の理解へ science.org/doi/10.1126/sc…

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CRISP_SCIENCE@ScienceCrisp·
毒素・抗毒素（TA）システムとも手を組んで侵入RNAに対抗するタイプIII-Bv CRISPR-Casシステムを発見 crisp-bio.blog.jp/archives/40594… （標的RNA切断 + cOA生合成を介したRNA破壊） ＋ 標的RNA存在下のみで毒素活性化. > 三重のディフェンスライン
CRISP_SCIENCE@ScienceCrisp

#CRISPR-Cas TYPE IIIーBv (interference stage) "Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin" Pandey S, Burman N [..] Wiedenheft B. Structure 2026-07-01. doi.org/10.1016/j.str.… ... Type III-B CRISPR-Cas variant (in Dissulfurispira thermophila) fused to MntA antitoxin and associated with a HEPN toxin. ... MetA adenylates HEPN toxin to be suppresed without target RNAs.

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CRISP_SCIENCE@ScienceCrisp·
#CRISPR-Cas TYPE IIIーBv (interference stage) "Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin" Pandey S, Burman N [..] Wiedenheft B. Structure 2026-07-01. doi.org/10.1016/j.str.… ... Type III-B CRISPR-Cas variant (in Dissulfurispira thermophila) fused to MntA antitoxin and associated with a HEPN toxin. ... MetA adenylates HEPN toxin to be suppresed without target RNAs.
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CRISP_SCIENCE@ScienceCrisp·
#CRISPR-Cas TYPE IIIーBv (expression stage: crRNA maturaion) "Involvement of RNase J in CRISPR RNA maturation in the cyanobacterium Synechocystis sp. PCC 6803" Bilger R [..] Hess WR. microLife 2025-09-01. doi.org/10.1093/femsml… "The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR-Cas subtype III-Bv system" Behler J, Sharma K, Reimann V, Wilde A, Urlaub H, Hess WR. Nat Microbiol. 2018-02-05/Mar. doi.org/10.1038/s41564…
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CRISP_SCIENCE@ScienceCrisp·
"PTAB Reaffirms Broad Institute Priority in #CRISPR-Cas9 Interference No. 106, 115: What the Decision Signals for Biotech Innovation and Patent Strategy" R. Danny Huntington, Anh-Thu Lam. JDSPURA 2026-06-25. jdsupra.com/legalnews/ptab… ... The CVC- Broad CRISPR interference dispute will continue since CVC filed a Notice of Appeal at the Federal Circuit on May 26th.
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CRISP_SCIENCE@ScienceCrisp·
“Genomic evidence of ecological flexibility and cross-niche #CRISPR spacerome targeting phage-plasmid hybrids in Latilactobacillus curvatus” Ibrahim C Kurt [..] Fatih Ortakci [+7] BMC Genomics 2026-06-30. doi.org/10.1186/s12864… ... this species has experienced diverse interactions with mobile genetic elements across multiple ecological contexts.
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CRISP_SCIENCE@ScienceCrisp·
"CsWRKY41 regulates the PYL-PP2C module to promote adventitious root formation in cucumber under waterlogging stress" Qiming Hu [..] Xiaohua Qi, Xuewen Xu. [+5] Plant Physiol. 2026-06-26. doi.org/10.1093/plphys… ... #CRISPR/Cas9-mediated mutation of CsWRKY41 significantly impaired AR formation, whereas its overexpression enhanced it.
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"Modeling Hereditary Angioedema With Personalized EPSC-Derived Hepatocytes: A CRISPR-Validated Platform for Mutation-Specific Mechanisms and Therapeutic Innovation" Xueyan Liu [..] Philip Hei Li. Allergy. 2026-06-20. doi.org/10.1111/all.70… PBMC-expanded erythroblasts from 4 patients were reprogrammed into expanded potential stem cells (EPSCs) and further differentiated into hepatocyte-like cells (HLCs). Reciprocal #CRISPR repair and installation confirm mutation-specific causality.
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CRISP_SCIENCE@ScienceCrisp·
Editorial "Pig Kidney Xenotransplantation: Immune Insights From a Landmark Human Case" Zhouqi Tang, Fadi G. Lakkis. Am J Kidney Dis. 2026-06-18. doi.org/10.1053/j.ajkd…
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CRISP_SCIENCE@ScienceCrisp·
"Immune profiling in a living human recipient of a gene-edited pig kidney" Ribas GT, Cunha AF, Avila JP [..] Borges TJ, Riella LV [+15]. Nat Med. 2026-01-08. doi.org/10.1038/s41591…
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