CRISP_SCIENCE

"Restoring the potency of a neutralizing antibody via guided hypermutation with hyper-antibody editor HAE1" Yuhong Wang, Yuefeng Guo [..] Jiazhi Hu. Genome Res 2026-05-06. doi.org/10.1101/gr.281… GHM (Guided Hypermutation) - We engineer a hyper-antibody editor, HAE1, by integrating cytidine and adenine deaminases with a nicked, PAMless Cas9 variant, SpRY, to closely resemble the mutation spectrum of natural SHM (Somatic hypermutation). #CRISPR

"Exogenous PAM richer resonator effect unlocks CRISPR/Cas12a biosensor-in-microdroplet for ultrasensitive and multiplexed food safety-related DNA detection" Roumeng Wang, Yongpo Mao [..] Long Ma. Trends Biotechnol. 2026-04-23. doi.org/10.1016/j.tibt… - ePAM-R-dsDNA (exogenous PAM Richer dsDNA) - CC-Drop-OPT（CRISPR/Cas12a microDroplet Overcoming PAM restriction on Targets）

"#CRISPR－Cas9 CD33-deleted allogeneic hematopoietic cell transplantation with gemtuzumab ozogamicin maintenance in AML: a phase 1/2 trial" John F. DiPersio, Guenther Koehne, Nirali N. Shah et al. Nat Med 2026-05-12. doi.org/10.1038/s41591… NCT04849910：Allogeneic Engineered Hematopoietic Stem Cell Transplant (HCT) Lacking the CD33 Protein, and Post-HCT Treatment With Mylotarg, for Patients With CD33+ AML or MDS (sponsor: Vor Biopharma) clinicaltrials.gov/study/NCT04849…

"A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish" Rebecca Wafer, Panna Tandon, James Minchin. eLife 2026-04-22. doi.org/10.7554/eLife.… ... Here, we integrate human transcriptomic data with a quantitative CRISPR-imaging platform in zebrafish to identify regulators of adipose morphology.

“Students Invented a New Diagnostic for Lyme Disease ― and a Tool for #CRISPR Researchers" Emily P. Bentley. Addgene Blog. 2026-05-12. blog.addgene.org/students-inven… Lambert-GA: Diagnostic Animation (2025) video.igem.org/w/gSwUYs3uMjFL… CASPER (Combined Amplification & Spacer Engine for RPA-Cas12a)

米、41人が「アンデス型」ハンタウイルス感染可能性　監視対象に＝保健当局 Ahmed Aboulenein, Michael Erman Reuters 2026-05-15. jp.reuters.com/world/security… 12州に乗客 25人と非乗客 16人

"Determining optimal sgRNA coverage and screening duration for pooled #CRISPR screens: A quantitative framework" Tao Xu, Zhenju Guo, Yinghao Li, Hongxia Lyu. Methods. 2026-05-08. doi.org/10.1016/j.ymet… ... we identify 15 days and 800 × coverage as the optimal screening parameters for standard iBAR‑based CRISPR screens.

"Anti-CRISPR Protein Regulates #CRISPR/Cas12a Fusogenic-Nanovesicle-Based Platform for Extracellular Vesicle-Encapsulated Non-Nucleic Acid Target In-Vesicle Detection" Qiaoxuan Zhang [..] Qiqin Wang, Xianzhang Huang. Angew Chem Int Ed Engl. 2026-05-07. doi.org/10.1002/anie.6… ... locked-activated CRISPR-Cas12a-AcrVA1 (LACA)

"A synthetic guide RNA scaffold enhanced #CRISPR/Cas9 editing efficiency in plants across multiple gene targets" Tushar K Dutta [..] Viswanathan Chinnusamy. Int J Biol Macromol. 2026-05-07. doi.org/10.1016/j.ijbi… ... Current study addresses the limitations of commonly used gRNA scaffold architecture by incorporating a stabilized stem-loop RAR (tetra loop) extension and a transcription-termination site mutation, resulting in improved RNA folding, increased Cas9 binding affinity, and enhanced in vivo editing outcomes.

REVIEW "Fourth Generation Gene Editors: Integration-Based Genome Engineering" Aidan E. Kincaid, Alex W. Hewitt, Rajendra KC. Molecular Therapy Advances. 2026-05-07. doi.org/10.1016/j.omta… ... Recombinases (e.g. Bridge recombinase), DNA Transposons (e.g. #CRISPR-Associated Transposon), retrotransposons & recombinases

"Inflammatory responses following #CRISPR modification of the nuclear localisation sequence in endogenous interleukin-1 alpha" Christopher Hoyle [..] David Brough, Gloria Lopez-Castejon. Dis Model Mech 2026-04-13. doi.org/10.1242/dmm.05… ... Pro-IL-1α NLS mutation does not affect expression but reduces nuclear localisation.

"Selective Elimination of TP53 Mutant Cells by Transcript-Activated Chromatin Shredding" Jingkun Zeng [..] Jennifer A. Doudna bioRxiv 2026-05-09 (preprint). doi.org/10.64898/2026.… #CRISPR-Cas12a2

"RNA-triggered CRISPR-Cas12a2 Preferentially and Cooperatively Cleaves Collateral DNA" Hallmark T, Kunwar S [..] Jackson RN. bioRxiv 2025-01-05 (preprint) doi.org/10.1101/2025.0… Figure 1. Cas12a2 preferentially cleaves collateral DNA substrates.

"A new kind of #CRISPR could treat viral infection and cancer by shredding sick cells' DNA" University of Utah Health. 2026-05-06. healthcare.utah.edu/newsroom/news/… ... Besides treating viral infections and cancer, Liu also thinks the molecule’s cell-killing potential could be applied to treat neurodegenerative diseases by eradicating cells in the brain that produce toxins. Or the molecule could be used to reverse other aging-related diseases by killing sick cells that consume resources without contributing function.

"Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases" Mateusz Walter, Giada Finocchio, Seraina Oberli [..] Irma Querques . bioRxiv 2026-05-07 (preprint). doi.org/10.64898/2026.… Tn7016 system from Pseudoalteromonas sp. S983 (PseCAST); cryo-EM structures of PseTnsAB paired-end complexes

"Replication-competent SIVcpz #CRISPR screen identifies barriers to successful cross-species transmission" Qinya Xie, Qingxing Wang, Sabrina Noettger, Guillermo Gosálbez [..] Frank Kirchhoff. J Virol. 2026-05-07. doi.org/10.1128/jvi.00… ... Four independent transmission events of simian immunodeficiency viruses from chimpanzees and gorillas to humans gave rise to human immunodeficiency virus type 1, but only one led to the global AIDS pandemic.

"A critical assessment of aptamer and #CRISPR-Cas12a-based biosensors for small molecule detection" Oliver F. Brandenberg, Elisabeth M.-L. Janssen, Olga T. Schubert. npj Biosensing 2026-05-08. doi.org/10.1038/s44328…

"#CRISPR-PTM and CRISPR-VEIS: Multiplexed platforms for quantitative functional analysis of endogenous phosphosites" Simon Willaume [..] Claus S. Sørensen. bioRxiv 2026-05-09 (preprint). doi.org/10.64898/2026.… ... CRISPR-PTM is a multiplexed knock-in framework generating defined phosphosite variants with internal allelic markers, enabling precise relative fitness effects in pooled populations. ... CRISPR-VEIS (Visualisation of Edits In Situ) is an in situ mRNA-genotyping approach that directly links ...

Review "Beyond the catalysis of Trypanosoma cruzi trans-sialidases: structure, function, post-translational modifications, intrinsically disordered regions and use of #CRISPR/Cas9" Uc-Chuc MA [..] Acosta-Viana KY. Arch Microbiol. 2026-05-08. doi.org/10.1007/s00203…

REVIEW "#CRISPR-based technologies for large DNA insertions" Mary Gracen A. Fuller, Matthew Foley, Rodolphe Barrangou Trends Biotechnol. 2026-05-06. doi.org/10.1016/j.tibt…

"Targeting a conserved functional motif in the PDS gene enables efficient #CRISPR/Cas9 editing in banana" Jeyabharathy Chandrasekaran, Backiyarani Suthanthiram et al. Sci Rep. 2026-05-06. doi.org/10.1038/s41598… ... accomplshed tri-allelic editing

"Lipid tail heterogeneity enables organ-selective mRNA delivery for in vivo #CRISPR-Cas9 gene editing" Jing Liao, Ling Zeng, Zixi Zhang, Yingsen Tang [..] Jinjin Chen. Materials Today. 2026-05-06/Aug. doi.org/10.1016/j.matt… HETAIL LNP (heterogeneous tail LNP) is capable of highly selective mRNA delivery to a broad spectrum of tissues, including the liver, lungs, spleen, lymph nodes, and bone marrow.