CRISP_SCIENCE

"Omega-based mini prime- and base-editing systems in Escherichia coli " Liu LH, Zhang Y, Xu B, Li Y, Guo Y, Lu X, Zhang Z [..] Wang S, Jiang A. Trends Biotechnol. 2026-03-16. doi.org/10.1016/j.tibt… OMEGA (Obligate Mobile Element Guided Activity): OMEGA-PE &OMEGA-ABE

"Self-inactivating AAV-CRISPR at different ages enables sustained amelioration of Huntington’s disease deficits in BAC226Q mice" Dai Y, Abudujielili Z [..] Zheng S, Li C. Sci Adv. 2026-03-18. doi.org/10.1126/sciadv… … a self-inactivating system that carries AAV-SaCas9-HTTg1 and an additional AAV-sgRNA to target Cas9 (gCas9), so that Cas9 sequence will be destroyed after HTT disruption.

"Modifying meiotic recombination by targeting chromatin regulators to crossover hotspots in Arabidopsis" Szymanska-Lejman M, Dziegielewski W, Wilhelm A, Hus K, Ziolkowski PA. Sci Adv 2026-03-13. doi.org/10.1126/sciadv… ... CRISPR: dCas9-JMJ14; dCas9-VP64

“Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis" Deol KK [..] Olzmann JA. Nat Struct Mol Biol. 2026-03-13. doi.org/10.1038/s41594… … we conducted CRISPR–Cas9 screens using a genome-edited, dual-fluorescent FSP1 reporter cell line, identifying both transcriptional and post-translational mechanisms that determine FSP1 levels.

Review "Construct design for precise DNA insertion in plants." Muzaffar A, Islam MT, Guss AM, Tuskan GA, Chen JG, Yang X. Trends Plant Sci. 2026-03-17. doi.org/10.1016/j.tpla…

"In vivo site-specific engineering to reprogram T cells" Nyberg WA, Bernard PL [..] Eyquem J. Nature. 2026-03-18. doi.org/10.1038/s41586… ... In vivo CAR-T cell therapy Scientists Create Cancer-Fighting Immune Cells Right in the Body" Williams SCP. Innovative Genomics Institute. 2026-03-18. innovativegenomics.org/news/in-vivo-c… NEWS "CRISPR makes enhanced cancer-fighting immune cells inside mice" Ledford H. Nature 2026-03-18. doi.org/10.1038/d41586… News & Views "A gene-editing method generates immunotherapeutic CAR T cells in the body" Holt R, Evgin L. Nature 2026-03-18. doi.org/10.1038/d41586…

"AcrIIA7 hijacks tracrRNA to block CRISPR-Cas system" Lee SY, Park HH. Nat Commun. 2026-03-14. doi.org/10.1038/s41467… ... Phocaeicola dorei AcrIIA7; PDB 9KZr

"Characterization and engineering of highly efficient Cas12j genome editors" Gundra SR, Jiang W [..] Mahfouz M. Trends Biotechnol. 2026-03-13. New orthologues: Cas 12i-11 ~ Cas 12j-18 doi.org/10.1016/j.tibt… … 8 new orthologues from 15,722,824 viral genomes 　Cas 12i-11 ~ Cas 12j-18 　718 aa ~ 796 aa 　5′-TBN-3′ (B=G/T/C) PAM … dsDNA break：T5Exo + Cas12i-12, -13, -18 … 5’-TAC tri-nucleotide in the target sequence enable robust editing efficiency … ABE: (d)Cas12i-13 + TadA8e

"A Single-Enzyme Activated CRISPR-Cas12a Nano System via Subtly Balanced dsDNA for Kinetic-Gated UDG Detection and Spatiotemporal Cellular Imaging" Dong K [..] Xiao X, Fu Q, Wang H. Adv Sci (Weinh). 2026-03-07. doi.org/10.1002/advs.2… … This dsDNA serves as a kinetic gatekeeper that maintains Cas12a in an inert state until UDG-mediated uracil excision disrupts the balance, lowering the energy barrier for crRNA invasion and initiating Cas12a trans-cleavage. … The system achieves a 1840-fold discrimination ratio and an ultralow detection limit of 5 × 10−7 U/mL.

"CRISPR screens establish regulatory maps of immunosuppressive surface molecules in cancer" Kalis R, Deswal S [..] Zuber J. bioRxiv 2026-03-11 (preprint). doi.org/10.64898/2026.… … To enable a more systematic, truly genome-wide analysis of regulatory pathways, we combined a doxycycline (Dox)-inducible Cas9 expression system14 and a second-generation sgRNA library15 to perform a series of time-controlled FACS-based CRISPR screens. … Our screens identify the membrane-trafficking factor DNAJC13 as a regulator of PD-L1 and CD276.

"In Vivo CRISPR Screening Identifies the Glutamate Receptor GRIA2 as Promoting Peritoneal Metastasis of Gastric Cancer via Calcium-Dependent β-Catenin Activation" Sun J [..] Zhao J, Ruan Y, Li H, Wang X. Adv Sci (Weinh). 2026-03-10. doi.org/10.1002/advs.2… … in both cell line-derived and patient-derived organoid xenograft (PDOX) mouse models.

"CRISPR-driven texture engineering: Redefining functional foods for precision nutrition and human health" Liberty JT, Alsalman FB, Lin H, Zhao X. J Funct Foods. 2026-03-12. doi.org/10.1016/j.jff.… … CRISPR enables genetic control of texture-relevant starch, protein, and cell-wall structures.

"In vitro and in vivo base editing of CCR5 in hematopoietic stem cells confers HIV-1 resistance" Anderson AK [..] Li C. Mol Ther. 2026-03-13. doi.org/10.1016/j.ymth… ... HDAd-BE

News Release "Simple ‘cocktail’ of amino acids dramatically boosts power of mRNA therapies and CRISPR gene editing" BIOHUB. EurekAlert! 2026-03-11. eurekalert.org/news-releases/… "Amino acid supplementation enhances in vivo efficacy of lipid nanoparticle–mediated mRNA delivery in preclinical models" Chen K, Wang W [..] Kelley SO, Wang Z. Sci Transl Med 2026-03-11. doi.org/10.1126/scitra…

"Inhalable Lipid Nanoparticles for Macrophage-Specific STING Gene Editing to Ameliorate Pulmonary Fibrosis" Wang Y [..] Mao C, Liu Y. Mol Ther. 2026-03-06. doi.org/10.1016/j.ymth… ... CRISPR-Cas9； ... optimized formulation, mCas9/gSting@DOPS, demonstrated over 7-fold greater macrophage expression efficiency compared to commercial formulations and was engineered for precise in vivo Sting1 gene editing.

"Genetic recording and in situ readout of single-cell signaling memory" Hao K [..] Askary A. (bioRxiv 2024-10-12) at Chem Biol 2026-03-11. doi.org/10.1038/s41589… ... INSCRIBE (IN situ Single Cell Recording of signal Intensity as Barcode Edits) based on ABE & atiometric barcode readout is a scalable platform for genetic recording and in situ readout of signaling history in single cells, advancing quantitative analysis of cell-cell communication during development and disease.

"Associate toxin–antitoxin with CRISPR-Cas to harness (ATTACH) engineered microbes" Zhao H [..] Liu H, Li M. Nucleic Acids Res. 2026-03-12. doi.org/10.1093/nar/gk… ... CRISPR repressed Toxin-Antitoxin (CreTA) in Type I-F CRISPR system ... ATTACH, a kill switch that associates toxin–antitoxin with CRISPR-Cas to harness engineered microbes, is a robust microbial biocontainment system (MBS)

"Mechanistic basis for improved activity of Engineered AsCas12a" Jansson-Fritzberg L, Chica B [..] Lemercier N, Wolk S. Commun Biol. 2026-03-10. doi.org/10.1038/s42003… ... AsCas12a Ultra ... Renizgamglogene autogedtemcel (reni-cel) based on engineered AsCass12a (PDB PDB 9MK3)

タンパク質の折り畳みは, そのサイズが大きいほど速いように見える - 鎖状のペプチド鎖が然るべき立体構造へと高速で (< 4 μs) 遷移する過程を蛍光可視化 crisp-bio.blog.jp/archives/40190…

AI企業の起業家がChatGPTに学びAlphaFoldも駆使してゲノム配列から設計し, 研究機関によって作製された「特注mRNAがんワクチン」が, 愛犬を救った物語 crisp-bio.blog.jp/archives/40193…