Sternberg Lab

11/11 Many thanks to all authors for their collective efforts in bringing this story to life! And a special thanks to @shsternberg and @IsraelF96135088 for their mentorship and support throughout this project.

10/11 This suggests a shared architectural logic underlying RNA-guided, tandem-repeat DNA synthesis across diverse bacterial Class 2 DRT systems & suggests that DRT10 is a structurally minimal version of the same strategy used by eukaryotic telomerase to achieve repeat addition.

1/11 New preprint from the Sternberg lab in collaboration with the Fernández lab! We are excited to share our structure-function study of DRT10, a bacterial defense-associated reverse transcriptase that synthesizes long tandem-repeat DNA.🧵 More here: biorxiv.org/content/10.648…

8/8 Want more? The preprint has it all-- subcomplex structures, mutagenesis validations & a clear path toward engineering next-gen gene insertion tools. Huge congrats to co-first authors Giada and Seraina, and the whole Jinek & Sternberg lab teams! 🎉 tinyurl.com/typeI-CAST

7/8 The full mechanistic model 🏛️: every step gated, every checkpoint structural. A complete blueprint for engineering better gene insertion tools. 🛠️

1/8 🚨 New preprint from the @SternbergLab & Jinek labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.

Be sure to also check out the elegant concurrent work from Alex Gao's group: science.org/doi/10.1126/sc…

9/9 This was a huge team effort! Big thanks to Kanta, Junichiro, and the Nishimasu lab for adjusting their meeting schedules to collaborate across time zones. Equal gratitude goes out to the Sternberg lab for the discussions and shared curiosity that shaped this work!

1/9 New preprint from the Sternberg Lab in collaboration with the Nishimasu Lab! We uncover how the DRT3 antiphage immune system pairs two reverse transcriptases, one RNA-templated and one protein-templated, to build a double-stranded DNA effector. doi.org/10.64898/2026.…