Filippo Caschera

Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery sciencedirect.com/science/articl… #CellFree #SynBio

ADAPT-M: a workflow for rapid, quantitative in vitro measurements of enriched protein libraries nature.com/articles/s4146…

Development of a Microdroplet-Based Functional Genomic Screening Pipeline by Combination of DNA Nanoflowers and PURExpress Cell-Free Expression doi.org/10.1021/acssyn…

Uncertainty-Driven Experiment Design in Cell-Free Protein Synthesis with Bayesian Optimization | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…

High-throughput mutational analysis of F1-ATPase by integrated cell-free protein synthesis and single-molecule rotation assay onlinelibrary.wiley.com/doi/10.1002/pr…

Cell-Free Genetic Circuits: Extract and Template Preparation link.springer.com/protocol/10.10…

Horseradish peroxidase as an electrochemical reporter protein for cell-free biosensors academic.oup.com/synbio/article…

De Novo Biosynthesis of Valinomycin From Glucose Using In Vitro Reconstituted Hybrid Pathways | ChemBioChem …mistry-europe.onlinelibrary.wiley.com/doi/10.1002/cb…

Programming Metabolic Dependency in Synthetic Cells Under Resource Scarcity biorxiv.org/content/10.648…

self-replicating artificial module-genome that generates bacterial chromosome replication system in vitro academic.oup.com/nar/article/54…

Theoretical framework and experimental demonstration of sustainable in vitro regeneration of major translation factors EF-Tu and IF3 biorxiv.org/content/10.648…

Cell-free synthesis and characterization of Salmonella, Escherichia coli, and Shigella-specific bacteriophages link.springer.com/article/10.118…

Breaking News: For the first time, researchers have created a cell with most of the hallmarks of life. nyti.ms/3SD2U1K

Our paper "Active-learning-guided optimization of cell-free systems for genome-wide transcriptomic profiling reveals progressive layers of regulation" is online @NatureComms ! Congrats @LeaWagnerSynBio, very proud of this work! doi.org/10.1038/s41467… #cellfree #AI #T7Phage

Active-learning-guided optimization of cell-free systems for genome-wide transcriptomic profiling reveals progressive layers of regulation nature.com/articles/s4146…

High-Throughput FRET Affinity Screening Technique (HTFAST) For Cell-Free Expressed Binding Protein Characterization biorxiv.org/content/10.648…

Tardigrade-Derived Strategy for Low-Cost Storage of Cell-Free Expression Lysates | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…

Phosphoserine as an Alternative Energy Source for E. coli Cell-Free Protein Synthesis with Increased Yield and Prolonged Activity pubs.acs.org/doi/10.1021/ac…

Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors pubs.acs.org/doi/10.1021/ac…

Field-deployable, lyophilized cell-free biosensor for robust and selective detection of inorganic arsenic in complex seaweed matrices sciencedirect.com/science/articl…

Nucleotide-Level Chemical Reaction Network Modeling Enables Quantitative Prediction of Reconstituted Cell-Free Expression Systems pubs.acs.org/doi/10.1021/ac…