WIBRFlow

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WIBRFlow

WIBRFlow

@WIBRFlow

Flow Cytometry Core Facility @ The Whitehead Institute for Biomedical Research

Cambridge, MA Katılım Nisan 2021
99 Takip Edilen192 Takipçiler
WIBRFlow
WIBRFlow@WIBRFlow·
Still not too late to register! We'll be back at the instrument today (in 1 hour) to look at cell cycle by flow cytometry! Looking forward to seeing everyone.
DerekDaviesCytometry@DDCytometry

Todays the day! The first OpenFlow Cytometry webinar of 2022. At 18:00GMT join @FlowMskcc and @WIBRFlow and me as we look at DNA analysis by #flowcytometry - theory and practical - come armed with questions. Still not too late to register: crick.zoom.us/webinar/regist…

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WIBRFlow
WIBRFlow@WIBRFlow·
The first #flowpostit of 2022 (in collaboration w/ @FlowMskcc) is here! Have you ever wondered about why laser delays are so important in your #flowcytometry experiments? Check out⬇️to get more information on what exactly these delays are and how they can impact your experiments!
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WIBRFlow
WIBRFlow@WIBRFlow·
Wishing all of our flow friends near and far a very happy holiday season!
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Whitehead Institute
Whitehead Institute@WhiteheadInst·
Whitehead Institute's Whitehead Fellows Program gives early-career scientists the rare opportunity to set up their own research labs as an alternative to traditional postdoc positions. Applications are open now until February 1st — apply today: fellowsearch.wi.mit.edu
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WIBRFlow
WIBRFlow@WIBRFlow·
Our next Flow Post-it in collaboration with MSKCC is out! Have you ever seen spillover between fluorochromes you weren't expecting? This is a good resource to understand why it may be happening! Happy Flowing!
flowMSKCC@FlowMskcc

#flowpostit Cross Laser Excitation - what can we do about it? 👇 - great collaboration w/ @WIBRFlow more: fccf.mskcc.org/flow-post-its

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WIBRFlow
WIBRFlow@WIBRFlow·
@jessvazquez314 @GRFlowDoctor @cat_uoc (part 4) and here is a photo to show a similar circumstance and what the root cause was. Sorry for the long winded answers. Happy Flowing and good luck!!
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Dr. Jessica Vazquez
Dr. Jessica Vazquez@jessvazquez314·
Q for Aurora users: we’re consistantly seeing a super negative pop in multiple parameters. It turns out they are CD20+. We’ve already moved CD20 to a different color, made no difference. Might this be a B cell biology thing I’m missing? @GRFlowDoctor @cat_uoc #cytekaurora
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WIBRFlow
WIBRFlow@WIBRFlow·
@jessvazquez314 @GRFlowDoctor @cat_uoc (part 3) ..and look at an nxn plot of all fluors. See if there is a pattern of what is typically called "overcompensation" or "undercompensation" even though it's unmixing. You can then try and identify what the issue is through your controls as Laura outlines in her post.
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WIBRFlow
WIBRFlow@WIBRFlow·
@jessvazquez314 @GRFlowDoctor @cat_uoc (part 2) I suggest looking at your single color controls and doing an overlay to see if your controls are as bright or brighter than your experimental samples. If not, that's a problem! You can also look at this this data in a third party analysis software (or in SpectroFlo)...
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WIBRFlow
WIBRFlow@WIBRFlow·
@jessvazquez314 @GRFlowDoctor @cat_uoc (part 1) Chiming in here as well! @cat_uoc posted a great resource. Specifically I would be curious about (how Laura mentions) if your single color controls are as bright or brighter than your experimental sample. If not, it can lead to issues with unmixing.
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Alvaro Quintanal-Villalonga
Alvaro Quintanal-Villalonga@AlvaroQuintanal·
Great night having Spanish dinner with labmates. Happy to be in a lab with such good people and to be able to share sangria with them. Thanks, @charlesrudin for taking us out!
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WIBRFlow
WIBRFlow@WIBRFlow·
Don't forget! This Friday along with @CrickTraining & @FlowMskcc we will be giving a free course on panel design, incorporating both theory and practice through data analysis! Free and open to all.
WIBRFlow@WIBRFlow

Join @CrickTraining @FlowMskcc and us next week on 11/19 as we continue our discussion about panel design for flow cytometry! Free and open to all. Engagement from participants is always encouraged. Register here: bit.ly/3C5lmlD

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Keşfet

@FlowMskcc @CYTOMETRYWHIZ @jessvazquez314 @GRFlowDoctor @cat_uoc @AlvaroQuintanal @charlesrudin @elonmusk