Xiaochen Fan

@BlandineP84 The TM tissue is a very thin tissue in the anterior chamber and the TM cells are relatively rare. When I dissected the TM tissue, I need to let them proliferate for 1 month to get enough numbers. Their proliferation speed will increase with the passages.

@XiaochenFan1 are there a lot of these cells in the eye or are they relatively rare cells? (how many cells can you get from one eye?)

Trabecular meshwork progenitor cell, a potential treatment for primary open-angle glaucoma. #HLSPosterDay2020 #HLSLCMS

@BlandineP84 Yes, I think these cells are diverse. The TM cells we are working on is from the human donor eyes. We are interested in the behaviours of those progenitor cells in glaucoma conditions, so may try to collect some TM progenitor cells from glaucoma donors in the future studies

@XiaochenFan1 so do you think these cells are very diverse? Will the cells come straight from human patients or are there other models? will you be just looking at normal cells or compare to diseased?

@stephlharrison Hi Steph, thank you :). We normally dissect the TM tissue under the microscope. However, the TM tissue in aged and glaucoma donors is very thin and weak. I'm thinking about doing the cell staining before the dissection

@XiaochenFan1 Hi Xiaochen, well-presented poster and easy to follow. I like how you have presented the challenges of the study. How do you plan to address your second challenge that TM cultures are difficult to obtain from aged and glaucoma donors?

@Svgebn Thank you :)

@BlandineP84 Thank you :) The flow cytometry (with sorting function) could be a good choice to help to purify the TM progenitor cells and my group is planning to run the single-cell sequence analysis to help to identify the multiple cell types in TM cultures.

@XiaochenFan1 very nice poster: it is clear and I enjoyed reading it. How will you approach these challenges that you have set in the poster?

@simon_too I think the culture could affect the phenotype include the serum effect and also the culture plate. I'm planning the find serum-replacement culture medium and trying to use the suspension plate to culture my progenitor cells🙂

@XiaochenFan1 Very interesting! Will you be assessing the phenotype of TM cells that have been isolated in culture? Will culturing have affected the phenotype? If so, how will you discern between functionally important phenotypic markers and those that represent cell culture adaptation?

@simon_too Hi, thank you. it's a really good question. My group has recently done the RNA sequence analysis about the TM cells and their progenitor cells to see the gene expression and planned to do the single-cell analysis to identify the different cell type in the TM culture.