Phillip Kyriakakis

@OdedRechavi Why not do both? 😂

When are you more concerned about the future of your job, during relaxed periods at work or when you're super busy?

Single-shot 20-fold expansion microscopy (relevance: 80%) nature.com/articles/s4159… #biophotonics #biomedicaloptics

@ATinyGreenCell I find Threads to have more content I like. Not much science on any of these sites compared to the old Twitter though, so I use them all less.

I'll try my best to cross post on blusky, but nowhere can I better serve small science acounts, share broadly, and also get rapid feedback on my own work than on this hellsite. 99% of my income now comes from research contracts established here so I am indebted to this community.

@pdhsu 💯

americans use mm.dd.yy europeans use dd.mm.yy scientists use yy.mm.dd

@ATinyGreenCell Addgene usually excludes the gene of interest from the feature list/map. So annoying for users, super bad training data for AI.

I'm sorry but I have a hard time accepting this as a worthy advancement. Training on addgene plasmids, which many are running legacy vectors with a LOT of excess junk from back when we didn't have a choice is gonna inspire a lot of the same dated design conventions. Hard disagree

@HarmitMalik Man, i feel that way with so many fewer requests than you. I can’t imagine how you do it.

It is academic letter request season.

@ATinyGreenCell most likely sequencing. I usually Sanger sequence and then find the homopolymers are okay.

Honestly, I don't blame anyone, neither my Q5 or the nanopore flow cell pores for not figuring out wtf to do with this polyA stretch, lol. I'll keep the clone that reads closest to truth, knowing it's either a sequencing artifact or my Q5 said "this is enough A's for one run"...

@ATinyGreenCell I can also use my fluorometer! I can pick the bandwidth and wavelength. It is super easy to stick things in there!

@breakliquid Test with some of the LEDs i sent ya to prove it actually is real

A dear friend donated an old plate reader to the sucrose project and now spending my lunch break inspecting parts and seeing what software is needed. This will act as a good benchmark for my bespoke plate reader build next month. Powers on but screen is busted. Checkin ebay...

@bielleogy @ATinyGreenCell I do him most start off with some integrity and most bad actors learn to be bad. So there are many examples you hear about of people that go from bad to good. That may also be bc of negative bias in our stories and memories too.

There is no grand genius when it comes to biology. No predisposition for bioresearch greatness. No BioMozarts. No BioJordans. The challenges are too massive and any semblance of extreme competence comes from very hard work and a LOT of luck. Natural talent for bio is myth.

@ProteiosTech I’d love to learn more about it!

@breakliquid Great question Phillip! We use L-Lysine and NOT imidazole. We're having some grand opening promotions today, if you'd like to try them out for yourself!

Grand Opening starts this Monday! Our team has worked hard to bring innovative protein purification solutions that will transform your research! Stay tuned for steep promotions, free products , and exclusive behind-the-scenes content during. Sign up now! proteios.com/grandopening #ProteiosTechnology #GrandOpening #Biotech #ProteinResearch #Innovation #Proteins #Metalloproteins

@jwsal sure

You have to be kidding me: nature.com/articles/d4158…

@com_ecology @tomkXY One crazy part is that senior PIs have so much less to gain by a Nature paper (or another Nature paper!), but they keep up the system and expectations. Jr researchers are dependent on them for promotions in most places, or to even get funding and keep their jobs.

@breakliquid @tomkXY Agreed! not simple. Some journals "justify" their exorbitant APCs by saying - hey, it's s an investment in your career bc our journals "assure" jobs, promotion, recognition & grants. Those paying "bet" in such strategy & convince themselves in some ways that's ok! Troublesome!

@abcairns @MJCliffe * for the first six months, then 29,9% APR 😝

@haesleinhuepf Its a big part of the answer, but more specifically senior PIs need to not submit to Nature et al, and keep the manuscript as a preprint first. They are the ones that can usually afford these fees and they set the example.

@breakliquid The answer is preprints. 🔓

@tomkXY If only it was that simple. It takes many of the senior PIs to do that first and normalize it before others can do that and still get promoted.

@breakliquid Just boycott Springer-Nature and post preprints instead. We have the power.

@ambulanzen @LingfengGe “because our industry is, to a large extent, resilient to economic downturns as investment in research and learning continues.” Actually because its a monopoly, its resistant.