Małgorzata Figiel

I wish to thank all the co-authors from @IIMCB_Poland and from @hauryliuk lab at @lunduniversity ! @SOLARIS_science

E. coli DRT7 provides defense against a very broad range of phages and uses a direct defense mechanism. The escape mutants of phage lambda and Bas1 that overcame this protection carried mutations in genes encoding a DNA mimic and a putative phosphoesterase, respectively.

Excited to share our preprint exploring the structural, biochemical and in vivo properties of DRT7/UG10 reverse transcriptases which produce dsDNA using a combination of primase and polymerase activities and provide broad-spectrum antiphage defense! biorxiv.org/content/10.648…

@MariuszCzC @11_Shuv_52 I wish to thank all the coauthors of this study and our colleagues from Nowotny lab. We are also grateful to @IIMCB_Poland, @NCN_PL and @SOLARIS_science for making this project possible!

@MariuszCzC @11_Shuv_52 Finally, we deliver the first complete cryo-EM structure of a two-component recombinase composed of ICP8 annealase and UL12 alkaline nuclease. The complex was reconstituted using a model DNA with a dsDNA and 3′ ssDNA overhang.

I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus. biorxiv.org/content/early/…

@MariuszCzC @11_Shuv_52 A very similar DNA conformation is observed in the crystal structures of ICP8 with 14-mer ssDNA. ICP8 subunits form dimers with a two-fold symmetry, with their DNA-binding sites facing each other and the outward-rotated bases of bound DNAs form pairs in a flexible manner.

@MariuszCzC @11_Shuv_52 We determined a cryo-EM structure of BALF2 bound to ssDNA at 3.2 Å resolution. The visible fragment of DNA is accommodated in a positively charged cleft. In the first four nucleotides, the base edges face the protein, but the next three bases are rotated away from the protein.

We are very happy to share the preprint of our latest study on the DNA-binding proteins from Herpes Simplex Virus-1 and Epstein-Barr Virus! @IIMCB_Poland

Following up on our paper about AbiK and Abi-P2, we now present an in-depth characterization of the third Abi polymerase, AbiA, performed in collaboration with @hauryliuk lab and @DziembowskiLab. academic.oup.com/nar/advance-ar…

Great job, congratulations to Shivlee and Mariusz!

@NaveedJ01722071 @IIMCB_Poland @MarcinNowotny @gmichlew Great! You can apply via this website: bit.ly/3tegzul Looking forward to hearing from you!

@IIMCB_Poland @MarcinNowotny @drfigiel @gmichlew I am interested I have completed master degree in Biotechnology with specialization in Molecular biology my master thesis is on DNA barcoding of Ticks.

#Warsaw4Phd We are looking for PhD students interested in the following topics: 👉Structural studies of herpesvirus proteins involved in DNA replication @MarcinNowotny @drfigiel 👉RNA-Protein Interactions in Human Health and Disease @gmichlew More: bit.ly/3g5tOtV

@JacekJaworski10 @IIMCB_Poland @NCN_PL Thanks, Jacek! ☺️ I am super stoked about this project already!

@IIMCB_Poland @drfigiel @NCN_PL Congrats to Gosia👏👏👏 This is big thing especially knowing that success rate was so low this round. Good luck with research.

💥Congrats to @drfigiel from @IIMCB_Poland on getting new research grant financed by @NCN_PL on DNA replication process in herpesviruses‼ 👏💪This is a great success, we are so proud of! And herpesviruses - beware❗😉

@its_rkgupta @IIMCB_Poland @NCN_PL Thank you, Rishi!

@IIMCB_Poland @drfigiel @NCN_PL Many congratulations @drfigiel 🎊🎉💐🍾

➡We are starting recruitment for #Warsaw4PhD within @IIMCB_Poland ➡We offer the opportunity to join 2️⃣ unique projects under the guidance of outstanding experts: prof.@MarcinNowotny dr #MalgorzataFigiel & prof. @gmichlew 👉Recruitment details: bit.ly/3VCRmYX