annnnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
wilkinsonlab.bio
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Max Wilkinson
158 posts
Max Wilkinson
@maxewilkinson
keen on reverse transcription | also keen on spliceosomes | cryoEM dabbler Assistant Member @MSKCC Post-doc @MIT PhD @MRC_LMB BSc @Otago
Manhattan, NY Katılım Ekim 2016
190 Takip Edilen3K Takipçiler
The Wilkinson Lab is open for science! @MSKCancerCenter
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
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@OliBClarke @EMDB_EMPIAR @kimanius @SjorsScheres alternatively, people might just discover I'm a bit rubbish at processing cryoEM data 😬
But this dataset was definitely challenging for me, at least!
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PS if someone in the Boston area spots a spare printed copy in their department tearoom could they please steal it for me 😊😊🙏🙏 (steal one for yourself first of course)
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covercovercovercovercovercovercovercovercovercovercover this is terrifically exciting
(Also please dw, the central dogma is still very much intact, this is just a bit of a funky spin on it)
Science Magazine@ScienceMagazine
The central dogma of molecular biology states that genetic information flows from DNA and RNA to protein, with reverse transcription converting RNA to DNA. In the pursuit of understanding how bacteria defend themselves from viral infection, two groups have found alternative pathways to making genes from RNA that did not previously encode proteins. Learn more this week in Science: bit.ly/3ZO7tI1
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@WojtekGalej thanks Wojtek! It's what happens when a reverse transcriptase gets jealous of its cousin Prp8 having all the fun with the RNA
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Congrats Max, great work and an awesome animation!
Max Wilkinson@maxewilkinson
My favourite discovery ever has just come online. Can I please tell you about some seriously wacky molecular biology? The story starts with a reverse transcriptase that SOMEHOW defends bacteria from viruses. (👇 I recommend sound ON for the video 🎹) 1/
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@Bryce_Nickels Correct! The reconstituted promoter is a 100% perfect match to that consensus, spacing and all. It's a better match to the consensus than most natural promoters. (This definitely gave us more hope in the hypothesis early on!)
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@maxewilkinson Unless I am misinterpreting something, the system generates a DNA with a full consensus promoter TTGACA - 17 - TATAAT in many cases - chromosomal promoters with the full consensus are extremely rare
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My favourite discovery ever has just come online. Can I please tell you about some seriously wacky molecular biology? The story starts with a reverse transcriptase that SOMEHOW defends bacteria from viruses.
(👇 I recommend sound ON for the video 🎹)
1/
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@cunha_tristan really good question! At the moment, unclear. We haven't figured out how the protein is toxic yet
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@maxewilkinson Can you explain why more repetitions make it more toxic? It seems like it would either ball up or stretch out?
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Max Wilkinson retweetledi
Such a crazy discovery. I think this captures the reaction of the audience while watching @maxewilkinson present this at the Symposium on the Immune System of Bacteria.
Max Wilkinson@maxewilkinson
My favourite discovery ever has just come online. Can I please tell you about some seriously wacky molecular biology? The story starts with a reverse transcriptase that SOMEHOW defends bacteria from viruses. (👇 I recommend sound ON for the video 🎹) 1/
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Lots more in the paper (#cryoEM etc!)
doi.org/10.1126/scienc…
Big thanks to coauthors @_David_Li @algao8 Rhiannon Macrae and @zhangf
Please also check out @shsternberg @stephentang23 related work - we come up with similar models, but it'll be exciting to resolve the differences!
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(P.S. eternal thanks as ever to @bradyajohnston for his #MolecularNodes plugin to #Blender3D. It makes molecular animation so much fun)
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Nils's incredible work is finally out, amazing work on a multi-talented protein by a multi-talented postdoc! It was a joy to use Dari @kimanius 's new Blush algorithm in #RELION to solve the cryoEM structure of this 40 kDa RNA complex
Nils Birkholz@NilsBirkholz
👋🏻 I'm absolutely glee-ridden to finally see our study on the RNA- and DNA-binding anti-CRISPR repressor Aca2 published in @Nature. 🥳 This unassuming little helix–turn–helix protein packs quite the punch! 🧶👇🏻 rdcu.be/dNl45
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Phage anti-CRISPR control by an RNA- and DNA-binding helix–turn–helix protein
RELION 5.0の論文で出てきた「Blushで40 kDaのRNA結合タンパクが2.5 Åで解けた」ってのは、コイツの事だったのか
nature.com/articles/s4158…
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Max Wilkinson retweetledi
In our newest preprint,
• we explore the effects of synonymous genome recoding, and
• construct & troubleshoot a synthetic 57-codon E. coli genome using multi-omics, editing, and laboratory evolution.
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