Nick Mateyko

@HaotianGuo_qb There are definitely a lot more variables that could be explored (different library types, coverage, culture volume, colony density). I'd love to see any data anyone has on this topic, especially conflicting results

@nmateyko Emmm..we have seen some data that suggests otherwise...But in general, I think it's really worthy to dig deeper in this topic.

Do you really need to spread plasmid libraries on mountains of plates to get uniform growth, or can you just dump them in a flask and call it a day? We make huge plasmid libraries in the de Boer Lab, so we tested whether culture method really matters. 1/ biorxiv.org/content/10.110…

@nevillesanjana I didn't test larger volumes, but it would only take ~5 more generations to get from 10 mL to maxiprep volume so I imagine the result wouldn't change much. This liquid vs plate comparison with maxipreps shows similar results: media.addgene.org/data/67/85/964…

@nmateyko This is great to see — thank you for sharing these insights from culture wars! 😀 One question: Did you also try the liquid culture at a larger scale than mini (e.g. maxiprep culture volumes)? Does it perform similarly well?

Check out the bioRxiv preprint for more details! biorxiv.org/content/10.110…

Overall, it seems like liquid culture is generally the best choice, and you can forget about spreading and scraping piles of plates every time you make a plasmid library! 9/